PXD010438

PXD010438 is an original dataset announced via ProteomeXchange.

Title	Analysis of the fission yeast meiotic proteome reveals a global switch from anabolic to catabolic processes and extensive post-transcriptional regulation.
Description	Progression through meiosis in Schizosaccharomyces pombe is regulated by stage-specific gene expression and translation, changes in RNA stability, expression of anti-sense transcripts and also requires stage-specific, targetted proteolysis of regulatory proteins. We have used SILAC labelling to examine the relative levels of proteins in S. pombe as diploid cells undergo meiosis. We found that the relative level of 880 proteins changes at least two-fold at some stage of meiosis. Most of these proteins either increase or decrease in level during the meiotic divisions, while some show transient peaks of expression. The most notable changes are in the proteostasis network, which shows a significant increase in components involved in protein turnover concomitant to a decrease in proteins involved in ribosome biogenesis. There was also an increase in ESCRT III protein levels; biological analysis reveals a role for some ESCRT III components in chromosome segregation and spore formation. Correlation with previous studies of gene expression and ribosome occupancy through meiosis reveals that changes in steady state protein levels are mainly regulated post-transcriptionally.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:29.656.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Romain Hamelin
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-16 03:38:21	ID requested
1	2019-02-15 02:41:40	announced
⏵ 2	2024-10-22 04:46:30	announced	2024-10-22: Updated project metadata.

Krapp A, Hamelin R, Armand F, Chiappe D, Krapp L, Cano E, Moniatte M, Simanis V, pombe Meiotic Proteome Reveals a Switch from Anabolic to Catabolic Processes and Extensive Post-transcriptional Regulation. Cell Rep, 26(4):1044-1058.e5(2019) [pubmed]

10.1016/j.celrep.2018.12.075;

curator keyword: Biological

submitter keyword: S. pombe, mass spectrometry, SILAC, time course, meiosis

Viesturs Simanis
contact affiliation	EPFL SV ISREC UPSIM SV2.1830 Station 19 CH - 1015 Lausanne Switzerland
contact email	viesturs.simanis@epfl.ch
lab head
Romain Hamelin
contact affiliation	PCF
contact email	romain.hamelin@epfl.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010438
     1. Label: PRIDE project
     2. Name: Analysis of the fission yeast meiotic proteome reveals a global switch from anabolic to catabolic processes and extensive post-transcriptional regulation.