PXD010433

PXD010433 is an original dataset announced via ProteomeXchange.

Title	The Antarctic Chlamydomonas spp. UWO241 and ICE-MDV exhibit differential restructuring of Photosystem I in response to iron
Description	Past studies have shown that the photosynthetic apparatus of a psychrophilic alga C. sp. UWO241 is remodeled to support high rates of Photosystem I (PSI)-associated cyclic electron flow (CEF). Iron levels in ELB are in the nanomolar range; therefore, we hypothesized that PSI restructuring in C. sp. UWO241 may reflect a strategy to survive long-term Fe-deficiency. We studied the effect of Fe availability in C. sp. UWO241, a mesophile, C. reinhardtii, and a second ELB psychrophile, Chlamydomonas sp. ICE-MDV. Under Fe-deficient conditions (2 µM Fe), abundance of the PSI reaction center protein, PsaA, as well as levels of photooxidizable P700 (ΔA820/A820) were significantly reduced in both psychrophiles relative to C. reinhardtii. In response to increased Fe, C. sp. ICE-MDV exhibited increases in PSI 77K Chl a fluorescence and ΔA820/A820 which matched that of C. reinhardtii, while C. sp. UWO241 exhibited moderate restoration of PSI function. Our results indicate that PSI functional organization in C. sp. UWO241 is unique. The unique physiological traits in PSI photochemistry exhibited by the psychrophiles may reflect adaptation to the stratified physicochemical conditions present the shallow vs. deep photic zones of a chemically Antarctic lake.
HostingRepository	PRIDE
AnnounceDate	2019-03-15
AnnouncementXML	Submission_2019-03-15_00:28:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	XIN WANG
SpeciesList	scientific name: Chlamydomonas sp. UWO 241; NCBI TaxID: 1653778;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-16 02:05:22	ID requested
⏵ 1	2019-03-15 00:28:09	announced

Cook G, Teufel A, Kalra I, Li W, Wang X, Priscu J, Morgan-Kiss R, The Antarctic psychrophiles Chlamydomonas spp. UWO241 and ICE-MDV exhibit differential restructuring of photosystem I in response to iron. Photosynth Res, 141(2):209-228(2019) [pubmed]

curator keyword: Biological

submitter keyword: iron, Photosystem I, Antarctica, psychrophile

Xin Wang
contact affiliation	Department of Microbiology Miami University, Oxford OH 45056
contact email	wangx98@miamioh.edu
lab head
XIN WANG
contact affiliation	Miami University
contact email	wangx98@miamioh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010433
     1. Label: PRIDE project
     2. Name: The Antarctic Chlamydomonas spp. UWO241 and ICE-MDV exhibit differential restructuring of Photosystem I in response to iron