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PXD010430

PXD010430 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTetrahydrobiopterin modulates ubiquitin conjugation to UBC13/UBE2N and proteasome activity by S-nitrosation
DescriptionNitric Oxide (NO) is an intracellular signalling mediator, which affects many biological processes via the posttranslational modification of proteins through S-nitrosation. The availability of NO and NOS-derived reactive oxygen species (ROS) from enzymatic uncoupling are determined by the NO synthase cofactor Tetrahydrobiopterin (BH4). Here, using a global proteomics “biotin-switch” approach, we identified components of the ubiquitin-proteasome system to be altered via BH4-dependent NO signalling by protein S-nitrosation. We show S-nitrosation of ubiquitin conjugating E2 enzymes, in particular the catalytic residue C87 of UBC13/UBE2N, leading to impaired polyubiquitylation by interfering with the formation of UBC13~Ub thioester intermediates. In addition, proteasome cleavage activity in cells also seems to be altered by S-nitrosation, correlating with the modification of cysteine residues within the 19S regulatory particle and catalytic subunits of the 20S complex. Our results highlight the widespread impact of BH4 on downstream cellular signalling as evidenced by the effect of a perturbed BH4-dependent NO-Redox balance on critical processes within the ubiquitin-proteasome system (UPS). These studies thereby uncover a novel aspect of NO associated modulation of cellular homeostasis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:46:27.238.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD010430
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRoman Fischer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListDeamidated; Oxidation; DeStreak; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-13 07:35:27ID requested
12018-10-02 07:20:48announced
22019-02-12 07:36:42announcedUpdated publication reference for PubMed record(s): 30254268.
32024-10-22 04:46:29announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41598-018-32481-4;
Bailey J, Davis S, Shaw A, Diotallevi M, Fischer R, Benson MA, Zhu H, Brown J, Bhattacharya S, Kessler BM, Channon KM, Crabtree MJ, Tetrahydrobiopterin modulates ubiquitin conjugation to UBC13/UBE2N and proteasome activity by S-nitrosation. Sci Rep, 8(1):14310(2018) [pubmed]
10.6019/PXD010430;
Keyword List
curator keyword: Biological
submitter keyword: biotin switch,S-nitrosylation
Contact List
Roman Fischer
contact affiliationHead of Discovery Proteomics Facility, Oxford
contact emailroman.fischer@ndm.ox.ac.uk
lab head
Roman Fischer
contact affiliationUniversity of Oxford
contact emailroman.fischer@ndm.ox.ac.uk
dataset submitter
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Dataset FTP location
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