PXD010425

PXD010425 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of HT1080 fibrosarcoma cells of mesenchymal or amoeboid migratory phenotype
Description	Amoeboid and mesenchymal migration of cancer cells both contribute to metastatic spreading of tumors. To characterize proteome changes underlying the different migratory modes, we performed mass spectrometry profiling of HT1080 fibrosarcoma cells undergoing mesenchymal-amoeboid transition induced by either doxycycline-inducible constitutively active RhoA or dasatinib treatment. Cells were kept in three-dimensional collagen gels with or without induction for 48 hours, lysed and processed for mass spectrometry analysis. In case of the inducible RhoA system, we also performed parallel two-dimensional experiments with cells on top of a dense layer of collagen (it was not feasible with dasatinib treatment).
HostingRepository	PRIDE
AnnounceDate	2020-04-23
AnnouncementXML	Submission_2020-07-15_00:09:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-13 05:29:30	ID requested
1	2020-04-23 06:39:41	announced
⏵ 2	2020-07-15 00:09:23	announced	2020-07-15: Updated publication reference for PubMed record(s): 32461585.

Č, erm, á, k V, Gandalovi, č, ov, á A, Merta L, Harant K, R, ö, sel D, Br, á, bek J, High-throughput transcriptomic and proteomic profiling of mesenchymal-amoeboid transition in 3D collagen. Sci Data, 7(1):160(2020) [pubmed]

curator keyword: Biological

submitter keyword: fibrosarcoma, Human, amoeboid, cell migration ,

Jan Brábek
contact affiliation	Charles University in Prague Faculty of Science Department of Cell Biology
contact email	jan.brabek@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD010425

PRIDE project URI

• PRIDE
  1. PXD010425
     1. Label: PRIDE project
     2. Name: Proteomic profiling of HT1080 fibrosarcoma cells of mesenchymal or amoeboid migratory phenotype