PXD010424

PXD010424 is an original dataset announced via ProteomeXchange.

Title	Identification of PWWP2A interactors using label-free quantitative affinity enrichment mass spectrometry
Description	Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified PWWP2A, which tightly binds to H2A.Z-containing nucleosomes and is involved in mitotic progression and cranial-facial development. Here, using in vitro assays we show that distinct domains of PWWP2A moreover mediate binding to free linker DNA as well as H3K36me3 nucleosomes. In vivo, PWWP2A strongly recognizes H2A.Z-containing regulatory regions and weakly H3K36me3-containing gene bodies. Additionally, PWWP2A bind to an MTA1-specific core NuRD (M1HR) complex solely consisting of MTA1, HDAC1 and RBBP4/7, excluding CHD and MBD proteins. Depletion of PWWP2A leads to an increase of acetylation levels on H3K27 as well as H2A.Z, presumably by impaired chromatin recruitment of M1HR. Thus, this study identifies PWWP2A as an ever more complex chromatin binding protein serving as adapter for M1HR to H2A.Z-containing chromatin, thereby promoting changes in histone acetylation levels and likely fine-tuning the transcriptional balance.
HostingRepository	PRIDE
AnnounceDate	2018-08-27
AnnouncementXML	Submission_2018-10-19_02:30:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Voelker-Albert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-13 04:30:48	ID requested
1	2018-08-27 07:23:04	announced
⏵ 2	2018-10-19 02:30:08	announced	Updated publication reference for PubMed record(s): 30327463.

Link S, Spitzer RMM, Sana M, Torrado M, V, ö, lker-Albert MC, Keilhauer EC, Burgold T, P, ü, nzeler S, Low JKK, Lindstr, ö, m I, Nist A, Regnard C, Stiewe T, Hendrich B, Imhof A, Mann M, Mackay JP, Bartkuhn M, Hake SB, PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nat Commun, 9(1):4300(2018) [pubmed]

curator keyword: Biological

submitter keyword: PWWP2A / H2A.Z / H3K36me3 / chromatin / NuRD / MTA1

Prof. Dr. Axel Imhof
contact affiliation	Histone Modifications Group Zentrallabor für Proteinanalytik BioMedical Center Faculty of Medicine Ludwig-Maximilians-University of Munich Großhadernerstr. 9 82152 Planegg-Martinsried
contact email	imhof@lmu.de
lab head
Moritz Voelker-Albert
contact affiliation	LMU Munich
contact email	Moritz.Voelker-Albert@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010424

PRIDE project URI

• PRIDE
  1. PXD010424
     1. Label: PRIDE project
     2. Name: Identification of PWWP2A interactors using label-free quantitative affinity enrichment mass spectrometry