PXD010412

PXD010412 is an original dataset announced via ProteomeXchange.

Title	The gut microbiota promotes hepatic fatty acid desaturation and elongation in mice
Description	Interactions between the gut microbial ecosystem and host lipid homeostasis are highly relevant to host physiology and metabolic diseases. We present a comprehensive multi-omics view of the effect of intestinal microbial colonization on hepatic lipid metabolism, integrating transcriptomic, proteomic, phosphoproteomic, and lipidomic analyses of liver and plasma samples from germfree and specific pathogen-free mice. Microbes induced monounsaturated fatty acid generation by stearoyl-CoA desaturase 1 and polyunsaturated fatty acid elongation by fatty acid elongase 5, leading to significant alterations in glycerophospholipid acyl-chain profiles. Germfree mice contained more abundant saturated and poly-unsaturated lipids, whereas colonized mice primarily contained mono-unsaturated lipids. A composite classification score calculated from the observed alterations in fatty acid profiles in germfree mice clearly differentiated antibiotic-treated mice from untreated controls with high sensitivity. Mechanistic investigations revealed that acetate originating from gut microbial degradation of dietary fiber serves as precursor for hepatic synthesis of C16 and C18 fatty acids and their related glycerophospholipid species that are also released into the circulation.
HostingRepository	PRIDE
AnnounceDate	2018-07-20
AnnouncementXML	Submission_2019-02-12_07:41:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominic Helm
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-13 00:46:12	ID requested
1	2018-07-20 02:50:44	announced
⏵ 2	2019-02-12 07:42:02	announced	Updated publication reference for PubMed record(s): 30218046.

Kindt A, Liebisch G, Clavel T, Haller D, H, ö, rmannsperger G, Yoon H, Kolmeder D, Sigruener A, Krautbauer S, Seeliger C, Ganzha A, Schweizer S, Morisset R, Strowig T, Daniel H, Helm D, K, ü, ster B, Krumsiek J, Ecker J, The gut microbiota promotes hepatic fatty acid desaturation and elongation in mice. Nat Commun, 9(1):3760(2018) [pubmed]

curator keyword: Biological

submitter keyword: Classification score, Data integration, Fatty acyls, Fatty acid signature, Lipidomics, Lipid metabolism, Microbiome, Short chain fatty acids, Stable isotope labelling

Josef Ecker
contact affiliation	Lehrstuhl für Ernährungsphysiologie, Technische Universität München (TUM), Gregor-Mendel-Str. 2, 85354 Freising, Germany; Phone: +498161-71-2385
contact email	josef.ecker@tum.de
lab head
Dominic Helm
contact affiliation	EMBL PCF
contact email	dominic.helm@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD010412

PRIDE project URI

• PRIDE
  1. PXD010412
     1. Label: PRIDE project
     2. Name: The gut microbiota promotes hepatic fatty acid desaturation and elongation in mice