PXD010409

PXD010409 is an original dataset announced via ProteomeXchange.

Title	Development of chemical proteomics for the folateome and analysis of the kinetoplastid folateome - Part 5
Description	In this current study, we aimed to produce and develop an affinity matrix for enzymes involved in the folate metabolism of kinetoplastids, effectively the “folateome”folateome of these parasites. This information will then be used to determine the molecular targets of compounds targeting folate metabolism, and to correlate this to phenotypic responses. To achieve this, a small library of clinical and literature anti-folate compounds were immobilized onto resins and used in “pull-down” experiments with and without test compound present, to ascertain proteins binding specifically to particular resins.8,10-11 While the initial focus of this project was to establish the kinetoplastid folateome, the approach has the potential to be extended to other organisms.
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2018-12-04_01:41:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Tinti
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-12 08:21:57	ID requested
1	2018-10-02 06:04:58	announced
⏵ 2	2018-12-04 01:41:37	announced	Updated project metadata.

Webster LA, Thomas M, Urbaniak M, Wyllie S, Ong H, Tinti M, Fairlamb AH, Boesche M, Ghidelli-Disse S, Drewes G, Gilbert IH, Development of Chemical Proteomics for the Folateome and Analysis of the Kinetoplastid Folateome. ACS Infect Dis, 4(10):1475-1486(2018) [pubmed]

curator keyword: Biological

submitter keyword: folateome folate kinetoplastids Trypanosoma Leishmania

Ian Gilbert
contact affiliation	Drug Discovery Unit Wellcome Centre for Anti-Infectives Research Division of Biological Chemistry and Drug Discovery University of Dundee Dundee, DD1 5EH. UK
contact email	i.h.gilbert@dundee.ac.uk
lab head
Michele Tinti
contact affiliation	Dundee University
contact email	m.tinti@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010409

PRIDE project URI

• PRIDE
  1. PXD010409
     1. Label: PRIDE project
     2. Name: Development of chemical proteomics for the folateome and analysis of the kinetoplastid folateome - Part 5