PXD010407 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Development of chemical proteomics for the folateome and analysis of the kinetoplastid folateome - Part 3 |
Description | In this current study, we aimed to produce and develop an affinity matrix for enzymes involved in the folate metabolism of kinetoplastids, effectively the “folateome”folateome of these parasites. This information will then be used to determine the molecular targets of compounds targeting folate metabolism, and to correlate this to phenotypic responses. To achieve this, a small library of clinical and literature anti-folate compounds were immobilized onto resins and used in “pull-down” experiments with and without test compound present, to ascertain proteins binding specifically to particular resins.8,10-11 While the initial focus of this project was to establish the kinetoplastid folateome, the approach has the potential to be extended to other organisms. |
HostingRepository | PRIDE |
AnnounceDate | 2018-12-04 |
AnnouncementXML | Submission_2018-12-04_01:41:15.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michele Tinti |
SpeciesList | scientific name: Leishmania major species complex; NCBI TaxID: 38581; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-12 08:06:19 | ID requested | |
1 | 2018-10-02 07:26:47 | announced | |
⏵ 2 | 2018-12-04 01:41:17 | announced | Updated project metadata. |
Publication List
Webster LA, Thomas M, Urbaniak M, Wyllie S, Ong H, Tinti M, Fairlamb AH, Boesche M, Ghidelli-Disse S, Drewes G, Gilbert IH, Development of Chemical Proteomics for the Folateome and Analysis of the Kinetoplastid Folateome. ACS Infect Dis, 4(10):1475-1486(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: folateome folate kinetoplastids Trypanosoma Leishmania |
Contact List
Ian Gilbert |
contact affiliation | Drug Discovery Unit Wellcome Centre for Anti-Infectives Research Division of Biological Chemistry and Drug Discovery University of Dundee Dundee, DD1 5EH. UK |
contact email | i.h.gilbert@dundee.ac.uk |
lab head | |
Michele Tinti |
contact affiliation | Dundee University |
contact email | m.tinti@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010407
- Label: PRIDE project
- Name: Development of chemical proteomics for the folateome and analysis of the kinetoplastid folateome - Part 3