PXD010404

PXD010404 is an original dataset announced via ProteomeXchange.

Title	A combination of proteomic approaches identifies a panel of circulating extracellular vesicle proteins related to the risk of suffering cardiovascular disease in obese patients
Description	Plasma-derived extracellular vesicles (EVs) have been extensively described as putative biomarkers in different diseases. More precisely, increased levels of EVs subpopulations are well-known to associate with obesity. The goal of this study was to identify EVs-derived biomarkers in plasma from obese patients in order to predict the development of pathological events associated with obesity. The study was performed combining two different proteomic approaches (2D-DIGE and label-free LC-MS/MS). Samples were obtained from 22 obese patients, with no associated comorbidities, and their lean-matched controls. EVs were isolated following a serial ultracentrifugation protocol. We detected 22 and 23 different regulated protein features from 2D-DIGE and label free LC-MS/MS respectively. Most of the differentially regulated proteins identified were involved in the coagulation and complement cascade. Interestingly, there was a clear up-regulation of complement C3, complement C4 and fibrinogen in obese patients following both approaches, which correlates with a pro-inflammatory and prothrombotic state of those individuals. On the other hand, we showed a down-regulation of adiponectin leading to an increased risk of suffering cardiovascular diseases. Our results suggest the relevance of considering plasma-derived-EVs proteins as a source of potential biomarkers for the development of atherothrombotic events in obesity.
HostingRepository	PRIDE
AnnounceDate	2018-12-17
AnnouncementXML	Submission_2018-12-17_05:54:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	María Núñez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-12 07:38:13	ID requested
⏵ 1	2018-12-17 05:54:51	announced

Barrachina MN, Sueiro AM, Casas V, Izquierdo I, Hermida-Nogueira L, Guiti, á, n E, Casanueva FF, Abi, á, n J, Carrascal M, Pardo M, Garc, í, a Á, A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients. Proteomics, 19(1-2):e1800248(2019) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Extracellular vesicles, Obesity, Plasma, Biomarkers, 2D-DIGE, Label-Free Quantitation

Maria N. Barrachina
contact affiliation	Platelet Proteomics Group. Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade Santiago de Compostela; Instituto de Investigación Sanitaria de Santiago (IDIS).
contact email	maria.nunez@usc.es
lab head
María Núñez
contact affiliation	University of Santiago de Compostela
contact email	maria.nunez@usc.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD010404

PRIDE project URI

• PRIDE
  1. PXD010404
     1. Label: PRIDE project
     2. Name: A combination of proteomic approaches identifies a panel of circulating extracellular vesicle proteins related to the risk of suffering cardiovascular disease in obese patients