PXD010389

PXD010389 is an original dataset announced via ProteomeXchange.

Title	Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate
Description	Staphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S.haemolyticus virulence factors is scarce. Bacterial virulence is mediated by membrane vesicles (MVs) which enable secretion and long distance delivery of bacterial effector molecules while protecting the cargo from proteolytic degradation from the environment. We wanted to determine if the MV protein cargo of S.haemolyticus is strain specific and enriched in certain MV associated proteins compared to the totalsecretome. The present study shows that both clinical and commensal S. haemolyticus isolates produce membrane vesicles. The MV cargo of both strains was enriched in proteins involved in adhesion and in acquisition of iron. The MV cargo of the clinical strain was further enriched in antimicrobial resistance proteins.
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2018-12-04_03:53:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack-Ansgar Bruun
SpeciesList	scientific name: Staphylococcus haemolyticus; NCBI TaxID: 1283;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-11 07:36:51	ID requested
1	2018-11-30 02:51:53	announced
⏵ 2	2018-12-04 03:53:40	announced	Updated project metadata.

Cavanagh JP, Pain M, Askarian F, Bruun JA, Urbarova I, Wai SN, Schmidt F, Johannessen M, Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate. J Proteomics, 197():106-114(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Membrane vesicles, Staphylococcus haemolyticus, secretome.

Jorunn Pauline Cavanagh
contact affiliation	1. Department of Paediatrics, University Hospital of North Norway, Tromsø, Norway 2 Paediatric Research Group, Department of Clinical Medicine, Faculty of Health Sciences, UiT- The Arctic University of Norway, Tromsø, Norway.
contact email	pauline.cavanagh@uit.no
lab head
Jack-Ansgar Bruun
contact affiliation	University of Tromsø
contact email	jack-ansgar.bruun@uit.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389

PRIDE project URI

• PRIDE
  1. PXD010389
     1. Label: PRIDE project
     2. Name: Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate