PXD010379

PXD010379 is an original dataset announced via ProteomeXchange.

Title	Exosomes and small microvesicles are key regulators of non-cell autonomous intercellular communication in senescence
Description	Senescence is a cellular phenotype present in health and disease, characterized by an irreversible cell cycle arrest and an inflammatory response, denominated senescence-associated secretory phenotype (SASP). The SASP is important in influencing the behaviour of neighbouring cells and altering the microenvironment; yet, this role has been mainly attributed to soluble factors. Here, we show that both the soluble factors and extracellular vesicles (EV) (comprised of exosomes and small extracellular vesicles) are capable of transmitting paracrine senescence to nearby cells. Analysis of the individual cells internalizing sEV, using a Cre-reporter system, suggest a positive correlation between sEV uptake from senescent cells and paracrine senescence. Interestingly, we find exosome biogenesis increased during senescence in vivo. sEV protein characterization by Mass Spectrometry (MS) followed by a functional siRNA screen identify the Interferon Induced Transmembrane Protein 3 (IFITM3) as partially responsible for transmitting senescence to normal cells. Altogether, we found that sEV are part of the SASP and contribute to paracrine senescence
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:45.521.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pilar Ximenez-Embun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-11 06:37:32	ID requested
1	2019-06-25 03:28:45	announced
2	2020-06-11 22:47:36	announced	2020-06-12: Updated publication reference for PubMed record(s): 31242426.
⏵ 3	2024-10-22 04:46:53	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2019.05.095;

Borghesan M, Fafi, á, n-Labora J, Eleftheriadou O, Carpintero-Fern, á, ndez P, Paez-Ribes M, Vizcay-Barrena G, Swisa A, Kolodkin-Gal D, Xim, é, nez-Emb, ú, n P, Lowe R, Mart, í, n-Mart, í, n B, Peinado H, Mu, ñ, oz J, Fleck RA, Dor Y, Ben-Porath I, Vossenkamper A, Mu, ñ, oz-Espin D, O'Loghlen A, Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3. Cell Rep, 27(13):3956-3971.e6(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: mass spectrometry

label free

exosomes

small extracellular vesicles

EV

paracrine senescence

OIS

DDIS

aging

tumour suppressor

IFITM3

Javier Munoz
contact affiliation	Proteomics Unit Spanish National Cancer Research Center (CNIO)
contact email	jmunozpe@cnio.es
lab head
Pilar Ximenez-Embun
contact affiliation	Proteomics Group
contact email	mpximenez@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD010379

PRIDE project URI

• PRIDE
  1. PXD010379
     1. Label: PRIDE project
     2. Name: Exosomes and small microvesicles are key regulators of non-cell autonomous intercellular communication in senescence