PXD010369

PXD010369 is an original dataset announced via ProteomeXchange.

Title	Spatial distribution of endogenous tissue protease activity in gastric carcinoma mapped by MALDI mass spectrometry imaging
Description	Aberrant protease activity has been implicated in the etiology of various prevalent diseases including neurodegeneration and cancer, in particular metastasis. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has recently been established as a key technology for bioanalysis of multiple biomolecular classes such as proteins, lipids and glycans. However, it has not yet been systematically explored for investigation of a tissue’s endogenous protease activity. In this study, we demonstrate that different tissues, spray-coated with substance P as a tracer, digest this peptide with different time-courses. Furthermore, we reveal that distinct cleavage products originating from substance P are generated transiently and that proteolysis can be attenuated by protease inhibitors in a concentration-dependent manner. Further we applied a photometrical protease activity assay to verify our results. To show the translational potential of the method, we analyzed protease activity of gastric carcinoma in mice. Our results reveal differential distribution of protease activity – with strongest activity being observed in mouse tumor tissue, suggesting the general applicability of the workflow in animal pharmacology and clinical studies.
HostingRepository	PRIDE
AnnounceDate	2018-10-25
AnnouncementXML	Submission_2018-10-25_06:32:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Torsten Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-11 01:51:27	ID requested
⏵ 1	2018-10-25 06:32:48	announced

Erich K, Reinle K, M, ü, ller T, Munteanu B, Sammour DA, Hinsenkamp I, Gutting T, Burgermeister E, Findeisen P, Ebert MP, Krijgsveld J, Hopf C, Spatial Distribution of Endogenous Tissue Protease Activity in Gastric Carcinoma Mapped by MALDI Mass Spectrometry Imaging. Mol Cell Proteomics, 18(1):151-161(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: SP3, LC-MS, MALDI, Gastric mouse carcinoma, Proteases

Prof. Dr. Carsten Hopf
contact affiliation	Mannheim University of Applied Sciences Center for Mass Spectrometry and Optical Spectroscopy (CeMOS)
contact email	carsten.hopf@medtech.uni-heidelberg.de
lab head
Torsten Mueller
contact affiliation	DKFZ
contact email	torsten.mueller84@googlemail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010369
     1. Label: PRIDE project
     2. Name: Spatial distribution of endogenous tissue protease activity in gastric carcinoma mapped by MALDI mass spectrometry imaging