PXD010364

PXD010364 is an original dataset announced via ProteomeXchange.

Title	Preeclampsia causes sex-specific transcriptional and proteomic changes in foetal erythroid cells
Description	Preeclampsia (PE) has been associated with placental dysfunction, resulting in foetal hypoxia, accelerated erythropoiesis and increased erythroblast count in the umbilical cord blood (UCB). Although the detailed effects remain unknown, placental dysfunction can also cause inflammation, nutritional and oxidative stress in the fetus that can affect erythropoiesis. Here, we compared the expression of surface adhesion molecules and erythroid differentiation capacity of UCB hematopoietic stem/ progenitor cells (HSPCs), UCB erythroid profiles along with transcriptome and proteome of these cells between male and female foetuses from PE and normotensive pregnancies. While no significant differences were observed in UCB HSPC migration/ homing and in vitro erythroid colony differentiation, the UCB HSPC transcriptome and the proteomic profile of the in vitro differentiated erythroid cells differed between PE vs normotensive samples. Accordingly, despite absence of significant differences in the UCB erythroid populations in male or female foetuses from PE or normotensive pregnancies, transcriptional changes were observed during erythropoiesis, particularly affecting male foetuses. Pathway analysis suggested deregulation in mTORC1/AMPK signaling pathways controlling cell cycle, differentiation and protein synthesis. These results associate PE with transcriptional and proteomic changes in foetal HSPCs and erythroid cells that may underlie the higher erythroblast count in the UCB in PE.
HostingRepository	PRIDE
AnnounceDate	2019-06-20
AnnouncementXML	Submission_2019-06-20_02:40:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Egor Vorontsov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-10 07:27:59	ID requested
⏵ 1	2019-06-20 02:40:19	announced

Masoumi Z, Maes GE, Herten K, Cort, é, s-Calabuig Á, Alattar AG, Hanson E, Erlandsson L, Mezey E, Magnusson M, Vermeesch JR, Familari M, Hansson SR, Preeclampsia is Associated with Sex-Specific Transcriptional and Proteomic Changes in Fetal Erythroid Cells. Int J Mol Sci, 20(8):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Preeclampsia, Hematopoietic stem/progenitor cells, Umbilical cord blood, Erythropoiesis, TMT, Q Exactive HF, LC-MS/MS

Zahra Masoumi
contact affiliation	Division of Obstetrics and Gynecology, Department of Clinical Sciences Lund, Lund University, Lund, Sweden
contact email	zahra.masoumi@med.lu.se
lab head
Egor Vorontsov
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact email	yegor.msu@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010364
     1. Label: PRIDE project
     2. Name: Preeclampsia causes sex-specific transcriptional and proteomic changes in foetal erythroid cells