PXD010354

PXD010354 is an original dataset announced via ProteomeXchange.

Title	In-depth evaluation of the acetylome in major human fungal pathogens elucidates the essential role of lysine acetylation in regulation of fungal pathogenicity
Description	Fungal pathogens cause deadly diseases in living organisms. Numerous studies suggest that lysine acetylation is a critical regulator in pathogenic fungi, but an in-depth and cross-species analysis of the acetylome in major pathogenic fungi is lacking, and our knowledge of the mechanism of acetylation in manipulating fungal pathogenicity is limited. Here, we show that lysine acetylation plays essential roles during cryptococcosis through modulating the gene expression of important virulence factors and the activity of key players in important pathways. We show that the Cryptococcus TOR pathway and translational elongation process are functionally regulated by acetylation via the HDAC and sirtuin families, respectively. Through comparative acetylome network analysis among pathogens and baker’s yeast, we demonstrate significant correlation between acetylation sites and virulence factors, indicating a unique selective pressure on lysine acetylation motifs in pathogenic fungi. These results provide a basis for understanding the regulation of fungal pathogenicity by posttranslational lysine acetylation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:11.539.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hai-Long Li
SpeciesList	scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: 235443;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-10 02:11:46	ID requested
1	2019-04-29 04:07:31	announced
⏵ 2	2024-10-22 04:46:12	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Cryptococcus neoformans, Virulence, Protein posttranslational modification,Human fungal pathogens, Acetylation

Cheney Ding
contact affiliation	College of Life and Health Sciences, Northeastern University, Shenyang, China
contact email	dingchen@mail.neu.edu.cn
lab head
Hai-Long Li
contact affiliation	Northeastern University
contact email	lihailong1166@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010354
     1. Label: PRIDE project
     2. Name: In-depth evaluation of the acetylome in major human fungal pathogens elucidates the essential role of lysine acetylation in regulation of fungal pathogenicity