PXD010352

PXD010352 is an original dataset announced via ProteomeXchange.

Title	Complementary proteomics strategies identify the ataxin-1 interactome in Neuro-2a cells
Description	To identify protein partners of ataxin-1 in neuronal cells under control or stress conditions, we use complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-trap pulldown) in Neuro-2a cells expressing epitope-tagged forms of ataxin-1[85Q]. These approaches allowed our enrichment of proximal proteins and interacting partners, respectively, with the subsequent protein identification performed by liquid chromatography-MS/MS. Background proteins, defined by identification not dependent on the polyQ-ataxin-1 protein, were additionally identified by their endogenous biotinylation (for the BioID protocol) or by their non-specific interaction with GFP only (in the GFP-trap protocol). All datasets were generated from biological replicates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:19.924.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sunyuan Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-10 01:53:50	ID requested
1	2018-10-18 00:57:48	announced
2	2019-02-12 07:44:18	announced	Updated publication reference for PubMed record(s): 30457570.
⏵ 3	2024-10-22 04:48:43	announced	2024-10-22: Updated project metadata.

10.1038/sdata.2018.262;

Zhang S, Williamson NA, Bogoyevitch MA, Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells. Sci Data, 5():180262(2018) [pubmed]

10.1038/s41467-020-17145-0;

Zhang S, Williamson NA, Duvick L, Lee A, Orr HT, Korlin-Downs A, Yang P, Mok YF, Jans DA, Bogoyevitch MA, The ataxin-1 interactome reveals direct connection with multiple disrupted nuclear transport pathways. Nat Commun, 11(1):3343(2020) [pubmed]

submitter keyword: GFP-trap,Ataxin-1, BioID, interactome, Neuro-2a, LC – MSMS

Marie A. Bogoyevitch
contact affiliation	Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria 3010, Australia
contact email	marieb@unimelb.edu.au
lab head
Sunyuan Zhang
contact affiliation	University of Melbourne
contact email	sunyuanz@student.unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010352

PRIDE project URI

• PRIDE
  1. PXD010352
     1. Label: PRIDE project
     2. Name: Complementary proteomics strategies identify the ataxin-1 interactome in Neuro-2a cells