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PXD010352

PXD010352 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComplementary proteomics strategies identify the ataxin-1 interactome in Neuro-2a cells
DescriptionTo identify protein partners of ataxin-1 in neuronal cells under control or stress conditions, we use complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-trap pulldown) in Neuro-2a cells expressing epitope-tagged forms of ataxin-1[85Q]. These approaches allowed our enrichment of proximal proteins and interacting partners, respectively, with the subsequent protein identification performed by liquid chromatography-MS/MS. Background proteins, defined by identification not dependent on the polyQ-ataxin-1 protein, were additionally identified by their endogenous biotinylation (for the BioID protocol) or by their non-specific interaction with GFP only (in the GFP-trap protocol). All datasets were generated from biological replicates.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:46:19.924.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSunyuan Zhang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-10 01:53:50ID requested
12018-10-18 00:57:48announced
22019-02-12 07:44:18announcedUpdated publication reference for PubMed record(s): 30457570.
32024-10-22 04:48:43announced2024-10-22: Updated project metadata.
Publication List
10.1038/sdata.2018.262;
Zhang S, Williamson NA, Bogoyevitch MA, Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells. Sci Data, 5():180262(2018) [pubmed]
10.1038/s41467-020-17145-0;
Zhang S, Williamson NA, Duvick L, Lee A, Orr HT, Korlin-Downs A, Yang P, Mok YF, Jans DA, Bogoyevitch MA, The ataxin-1 interactome reveals direct connection with multiple disrupted nuclear transport pathways. Nat Commun, 11(1):3343(2020) [pubmed]
Keyword List
submitter keyword: GFP-trap,Ataxin-1, BioID, interactome, Neuro-2a, LC – MSMS
Contact List
Marie A. Bogoyevitch
contact affiliationDepartment of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria 3010, Australia
contact emailmarieb@unimelb.edu.au
lab head
Sunyuan Zhang
contact affiliationUniversity of Melbourne
contact emailsunyuanz@student.unimelb.edu.au
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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