PXD010352 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Complementary proteomics strategies identify the ataxin-1 interactome in Neuro-2a cells |
Description | To identify protein partners of ataxin-1 in neuronal cells under control or stress conditions, we use complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-trap pulldown) in Neuro-2a cells expressing epitope-tagged forms of ataxin-1[85Q]. These approaches allowed our enrichment of proximal proteins and interacting partners, respectively, with the subsequent protein identification performed by liquid chromatography-MS/MS. Background proteins, defined by identification not dependent on the polyQ-ataxin-1 protein, were additionally identified by their endogenous biotinylation (for the BioID protocol) or by their non-specific interaction with GFP only (in the GFP-trap protocol). All datasets were generated from biological replicates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:46:19.924.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sunyuan Zhang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-10 01:53:50 | ID requested | |
1 | 2018-10-18 00:57:48 | announced | |
2 | 2019-02-12 07:44:18 | announced | Updated publication reference for PubMed record(s): 30457570. |
⏵ 3 | 2024-10-22 04:48:43 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/sdata.2018.262; |
Zhang S, Williamson NA, Bogoyevitch MA, Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells. Sci Data, 5():180262(2018) [pubmed] |
10.1038/s41467-020-17145-0; |
Zhang S, Williamson NA, Duvick L, Lee A, Orr HT, Korlin-Downs A, Yang P, Mok YF, Jans DA, Bogoyevitch MA, The ataxin-1 interactome reveals direct connection with multiple disrupted nuclear transport pathways. Nat Commun, 11(1):3343(2020) [pubmed] |
Keyword List
submitter keyword: GFP-trap,Ataxin-1, BioID, interactome, Neuro-2a, LC – MSMS |
Contact List
Marie A. Bogoyevitch |
contact affiliation | Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria 3010, Australia |
contact email | marieb@unimelb.edu.au |
lab head | |
Sunyuan Zhang |
contact affiliation | University of Melbourne |
contact email | sunyuanz@student.unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010352
- Label: PRIDE project
- Name: Complementary proteomics strategies identify the ataxin-1 interactome in Neuro-2a cells