PXD010345
PXD010345 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | AFFIRM_in-solution (Sall et al. 2018) |
Description | This dataset describes the use of biotin-tagged scFv and streptavidin coated magnetic beads for targeted protein captures in the immunoaffinity SRM platform AFFIRM. Proteins are captured using two setups, either incubating scFv and protein in-solution and after binding adding magnetic beads, or by incubating bead-coupled scFv in solution with target protein. Proteins are digested on-bead and analyzed with a peptide SRM readout. Dilution curves of target proteins in serum background from 1-125 ng/ml concentrations are analyzed. |
HostingRepository | PeptideAtlas |
AnnounceDate | 2018-07-09 |
AnnouncementXML | Submission_2018-07-09_09:41:13.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Sofia Waldemarson |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TSQ Vantage |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-07-09 09:39:42 | ID requested | |
⏵ 1 | 2018-07-09 09:41:15 | announced |
Publication List
S, ä, ll A, Corbee D, Vikstr, ö, m S, Ottosson F, Persson H, Waldemarson S, Advancing the immunoaffinity platform AFFIRM to targeted measurements of proteins in serum in the pg/ml range. PLoS One, 13(2):e0189116(2018) [pubmed] |
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted |
Contact List
Sofia Waldemarson | |
---|---|
contact affiliation | Immunotechnology Lund University |
contact email | sofia.waldemarson@immun.lth.se |
dataset submitter | |
Peter James | |
contact affiliation | Immunotechnology Lund University |
contact email | peter.james@immun.lth.se |
lab head |
Full Dataset Link List
PeptideAtlas dataset URI |
PASSEL experiment URI |
PASSEL transition group browser URI |