PXD010345

PXD010345 is an original dataset announced via ProteomeXchange.

Title	AFFIRM_in-solution (Sall et al. 2018)
Description	This dataset describes the use of biotin-tagged scFv and streptavidin coated magnetic beads for targeted protein captures in the immunoaffinity SRM platform AFFIRM. Proteins are captured using two setups, either incubating scFv and protein in-solution and after binding adding magnetic beads, or by incubating bead-coupled scFv in solution with target protein. Proteins are digested on-bead and analyzed with a peptide SRM readout. Dilution curves of target proteins in serum background from 1-125 ng/ml concentrations are analyzed.
HostingRepository	PeptideAtlas
AnnounceDate	2018-07-09
AnnouncementXML	Submission_2018-07-09_09:41:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sofia Waldemarson
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-09 09:39:42	ID requested
⏵ 1	2018-07-09 09:41:15	announced

S, ä, ll A, Corbee D, Vikstr, ö, m S, Ottosson F, Persson H, Waldemarson S, Advancing the immunoaffinity platform AFFIRM to targeted measurements of proteins in serum in the pg/ml range. PLoS One, 13(2):e0189116(2018) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

Sofia Waldemarson
contact affiliation	Immunotechnology Lund University
contact email	sofia.waldemarson@immun.lth.se
dataset submitter
Peter James
contact affiliation	Immunotechnology Lund University
contact email	peter.james@immun.lth.se
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI