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PXD010345

PXD010345 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAFFIRM_in-solution (Sall et al. 2018)
DescriptionThis dataset describes the use of biotin-tagged scFv and streptavidin coated magnetic beads for targeted protein captures in the immunoaffinity SRM platform AFFIRM. Proteins are captured using two setups, either incubating scFv and protein in-solution and after binding adding magnetic beads, or by incubating bead-coupled scFv in solution with target protein. Proteins are digested on-bead and analyzed with a peptide SRM readout. Dilution curves of target proteins in serum background from 1-125 ng/ml concentrations are analyzed.
HostingRepositoryPeptideAtlas
AnnounceDate2018-07-09
AnnouncementXMLSubmission_2018-07-09_09:41:13.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSofia Waldemarson
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTSQ Vantage
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-09 09:39:42ID requested
12018-07-09 09:41:15announced
Publication List
S, ä, ll A, Corbee D, Vikstr, ö, m S, Ottosson F, Persson H, Waldemarson S, Advancing the immunoaffinity platform AFFIRM to targeted measurements of proteins in serum in the pg/ml range. PLoS One, 13(2):e0189116(2018) [pubmed]
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted
Contact List
Sofia Waldemarson
contact affiliationImmunotechnology Lund University
contact emailsofia.waldemarson@immun.lth.se
dataset submitter
Peter James
contact affiliationImmunotechnology Lund University
contact emailpeter.james@immun.lth.se
lab head
Full Dataset Link List
PeptideAtlas dataset URI
PASSEL experiment URI
PASSEL transition group browser URI