PXD010344

PXD010344 is an original dataset announced via ProteomeXchange.

Title	PCa_EVs_SRM (Sequeiros et al. 2017)
Description	SUMMARY Rapid, reliable diagnosis of prostate cancer (PCa) is highly desirable as current methods for diagnosis lack sensitivity to successfully detect this type of cancer in early stages. In addition, identification of PCa biomarkers that can classify patients into high- and low-risk groups for disease progression may impact treatment decision-making and is a major area of on-going research. Here, we describe a set of protein-combination panels in urinary extracellular vesicles (EVs), defined by targeted proteomics and immunoblotting techniques, to improve early non-invasive detection and stratification of PCa patients. We report a two-protein combination in urinary EVs to classify benign and PCa patients (ADSV-TGM4; AUC= 0.652), and a combination of five proteins able to significantly distinguish between high- and low-grade PCa patients (CD63-GLPK5-SPHM-KLK3-PAPP; AUC=0.701). Immunohistochemistry assays on 98 benign prostatic versus 136 PCa tissues confirmed a strong link between the urinary EVs proteome and expression alterations in PCa tissues. ADSV and TGM4 expression yielded a high diagnostic potential (AUC 0.729 and 0.813) and promising TGM4 prognostic power between low and high-grade tumors (AUC 0.817) as well as detecting those patients with biochemical recurrence (AUC = 0.803). Our study highlights the potential of SRM as diagnostic assay for liquid biopsies via urinary EVs to improve diagnosis and prognosis of suspected PCa patients.
HostingRepository	PeptideAtlas
AnnounceDate	2018-07-09
AnnouncementXML	Submission_2018-07-09_09:41:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Meierhofer
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	QTRAP 5500

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-09 09:37:50	ID requested
⏵ 1	2018-07-09 09:41:04	announced

Sequeiros T, Rigau M, Chiva C, Montes M, Garcia-Grau I, Garcia M, Diaz S, Celma A, Bijnsdorp I, Campos A, Di Mauro P, Borr, ó, s S, Revent, ó, s J, Doll A, Paciucci R, Pegtel M, de Torres I, Sabid, ó E, Morote J, Olivan M, Targeted proteomics in urinary extracellular vesicles identifies biomarkers for diagnosis and prognosis of prostate cancer. Oncotarget, 8(3):4960-4976(2017) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

David Meierhofer
contact affiliation	Max Planck Institute for Molecular Genetics
contact email	meierhof@molgen.mpg.de
dataset submitter
David Meierhofer
contact affiliation	MPIMG
contact email	meierhof@molgen.mpg.de
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI