PXD010341

PXD010341 is an original dataset announced via ProteomeXchange.

Title	Optimising size exclusion chromatography for extracellular vesicle enrichment and proteomic analysis from clinically relevant samples
Description	We performed a quantitative evaluation and optimization of SEC for separating EVs from contaminating proteins. This optimized method was applied to enrich EVs from healthy plasma and MDA-MB-231 cancer cell culture medium. By spiking-in cancer cell-derived EVs to healthy plasma and then performing SEC enrichment, we were subsequently to detect cancer cell line- specific proteins by LC-MS/MS. We quantified the limit of detection and showed that cancer EV-associated proteins were detectable by nano-LC-MS/MS when as little as 1% of the total plasma EV number were derived from a cancer cell line.
HostingRepository	PRIDE
AnnounceDate	2019-01-16
AnnouncementXML	Submission_2019-01-16_02:08:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebecca Lane
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-09 08:10:56	ID requested
⏵ 1	2019-01-16 02:08:08	announced

Lane RE, Korbie D, Trau M, Hill MM, Optimizing Size Exclusion Chromatography for Extracellular Vesicle Enrichment and Proteomic Analysis from Clinically Relevant Samples. Proteomics, 19(8):e1800156(2019) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: Plasma, Extracellular Vesicle, Chromatography

Michelle M Hill
contact affiliation	QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, QLD Australia 4103
contact email	michelle.hill@qimrberghofer.edu.au
lab head
Rebecca Lane
contact affiliation	University of Queensland
contact email	r.lane2@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010341

PRIDE project URI

• PRIDE
  1. PXD010341
     1. Label: PRIDE project
     2. Name: Optimising size exclusion chromatography for extracellular vesicle enrichment and proteomic analysis from clinically relevant samples