PXD010340

PXD010340 is an original dataset announced via ProteomeXchange.

Title	Heat-adaptive proteome in filling wheat grain
Description	Wheat (T. aestivum L. Chinese Spring) plants were cultured in 25-cm buckets at a density of 16 plants per bucket in a greenhouse at a temperature of 15–30 °C. Plants were watered once a week, and artificial light was applied when there was fewer than 12 h of natural daylight. The flowering date of each spike was recorded when the first flower appeared on the spike. Comparable wheat plants were selected depending on the flowering time and growth status and were transferred into growth chambers 12 DAF for a three-day adaptation period to the growth chamber environment (24/17 °C, 14/10 h day/night cycle). Wheat plants were illuminated with a photosynthetic photon flux density of 360 µmol m˗2 s˗1 at canopy height, and relative humidity ranged from 40% to 70%. Half of the wheat plants (about 50 plants) were transferred to heated conditions (37/17 °C, 14/10 h day/night cycle, 40% to 70% humidity), while the others were maintained under the standard conditions described above as a control. After three day/night cycles, the heated samples were collected after the plants had been exposed to heat for 4 h, and the control samples from the unheated chambers were collected at the same time for and proteomic analyses. A minimum of 20 spikes were collected from each condition, immediately immersed in liquid nitrogen and stored at ˗80 °C until further analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:11.431.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010340
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yunze Lu
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-09 03:42:09	ID requested
1	2018-08-29 05:52:54	announced
2	2018-09-11 00:32:42	announced	Updated project metadata.
⏵ 3	2024-10-22 04:46:11	announced	2024-10-22: Updated project metadata.

10.6019/PXD010340;

curator keyword: Biological

submitter keyword: grain,heat stress,wheat,TMT proteomics

Shengbao Xu
contact affiliation	State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China
contact email	xushb@nwsuaf.edu.cn
lab head
Yunze Lu
contact affiliation	Northwest A&F University
contact email	lyz@nwsuaf.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010340
     1. Label: PRIDE project
     2. Name: Heat-adaptive proteome in filling wheat grain