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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-08-29
  • AnnouncementXML: Submission_2018-08-29_05:09:48.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD010336
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Howard Choi
  • Title: Integrated Dissection of Cysteine Oxidative Modification Proteome During Cardiac Hypertrophy
  • Description: Cysteine oxidative modification of cellular proteins is crucial for many aspects of cardiac hypertrophy development. However, integrated dissection of multiple types of cysteine oxidation proteome in cardiac hypertrophy is currently missing. Here we developed a novel discovery platform encompassing a customized biotin switch-based quantitative proteomics pipeline and an advanced analytic workflow to comprehensively profile the landscape of cysteine oxidation in ISO-induced cardiac hypertrophy mouse model. Specifically, we identified a total of 3,717 proteins containing 6,837 oxidized cysteine sites by at least one of reversible cysteine oxidation, cysteine sulfinylation (CysSO2H), and cysteine sulfonylation (CysSO3H). Analyzing the hypertrophy signatures that are reproducibly discovered from computational workflow highlighted a group of fatty acid beta-oxidation enzymes with a continual decreased temporal pattern and a significant decreased abundance in reversible oxidation with no temporal or abundance change in total cysteine, revealing the oxidative regulatory map of fatty acid metabolism in cardiac hypertrophy, which is featured by an overall reduced oxidative metabolism. Our cysteine oxidation platform depicts a dynamic and integrated landscape of the cysteine oxidative proteome, extracted molecular signature, and provided mechanistic insights in cardiac hypertrophy.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: biotin-maleimide; Dimethyl; NEM; Carbamidomethyl; 47.9947; Dimethyl:2H(4)
  • Instrument: LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-07-06 09:08:44ID requested
12018-08-29 05:09:50announced

Publication List

  1. Wang J, Choi H, Chung NC, Cao Q, Ng DCM, Mirza B, Scruggs SB, Wang D, Garlid AO, Ping P, Integrated Dissection of Cysteine Oxidative Post-translational Modification Proteome During Cardiac Hypertrophy. J Proteome Res, 17(12):4243-4257(2018) [pubmed]

Keyword List

  1. submitter keyword: mouse, heart, cysteine oxidation, reversible cysteine oxidation, cysteine sulfinylation, cysteine sulfonylation, cardiac hypertrophy, biotin switch, dimethyl labeling, quantitative proteomics

Contact List

    Peipei Ping
    • contact affiliation: David Geffen School of Medicine, UCLA, USA BD2K Center of Excellence for Big Data Computing at UCLA, UCLA, USA
    • contact email: pping38@g.ucla.edu
    • lab head:
    Howard Choi
    • contact affiliation: BD2K Center of Excellence for Big Data Computing at UCLA, UCLA, USA
    • contact email: cjh9595@g.ucla.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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