PXD010336

PXD010336 is an original dataset announced via ProteomeXchange.

Title	Integrated Dissection of Cysteine Oxidative Modification Proteome During Cardiac Hypertrophy
Description	Cysteine oxidative modification of cellular proteins is crucial for many aspects of cardiac hypertrophy development. However, integrated dissection of multiple types of cysteine oxidation proteome in cardiac hypertrophy is currently missing. Here we developed a novel discovery platform encompassing a customized biotin switch-based quantitative proteomics pipeline and an advanced analytic workflow to comprehensively profile the landscape of cysteine oxidation in ISO-induced cardiac hypertrophy mouse model. Specifically, we identified a total of 3,717 proteins containing 6,837 oxidized cysteine sites by at least one of reversible cysteine oxidation, cysteine sulfinylation (CysSO2H), and cysteine sulfonylation (CysSO3H). Analyzing the hypertrophy signatures that are reproducibly discovered from computational workflow highlighted a group of fatty acid beta-oxidation enzymes with a continual decreased temporal pattern and a significant decreased abundance in reversible oxidation with no temporal or abundance change in total cysteine, revealing the oxidative regulatory map of fatty acid metabolism in cardiac hypertrophy, which is featured by an overall reduced oxidative metabolism. Our cysteine oxidation platform depicts a dynamic and integrated landscape of the cysteine oxidative proteome, extracted molecular signature, and provided mechanistic insights in cardiac hypertrophy.
HostingRepository	PRIDE
AnnounceDate	2018-08-29
AnnouncementXML	Submission_2018-08-29_05:09:48.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010336
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Howard Choi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotin-maleimide; Dimethyl; NEM; Carbamidomethyl; 47.9947; Dimethyl:2H(4)
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-06 09:08:44	ID requested
⏵ 1	2018-08-29 05:09:50	announced

Wang J, Choi H, Chung NC, Cao Q, Ng DCM, Mirza B, Scruggs SB, Wang D, Garlid AO, Ping P, Integrated Dissection of Cysteine Oxidative Post-translational Modification Proteome During Cardiac Hypertrophy. J Proteome Res, 17(12):4243-4257(2018) [pubmed]

submitter keyword: mouse, heart, cysteine oxidation, reversible cysteine oxidation, cysteine sulfinylation, cysteine sulfonylation, cardiac hypertrophy, biotin switch, dimethyl labeling, quantitative proteomics

Peipei Ping
contact affiliation	David Geffen School of Medicine, UCLA, USA BD2K Center of Excellence for Big Data Computing at UCLA, UCLA, USA
contact email	pping38@g.ucla.edu
lab head
Howard Choi
contact affiliation	BD2K Center of Excellence for Big Data Computing at UCLA, UCLA, USA
contact email	cjh9595@g.ucla.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010336
     1. Label: PRIDE project
     2. Name: Integrated Dissection of Cysteine Oxidative Modification Proteome During Cardiac Hypertrophy