PXD010333 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of intact mannose-6-phosphate glycopeptides |
Description | We have repurposed standard phosphoproteomics workflow based on Fe3+IMAC for enrichment ofmannose-6-phosphate modified glycopeptides. This worklfow was used to profile lysosomal acid hydrolases in HeLa and CHO cell lines. We have combined this approach with CRISPR/Cas9 KO of acid phosphatases 2 and 5 responsible for dephosphorylation of mannose-6-phosphate glycopeptides in the lysosome. This combined approach enabled significantly deeper coverage of CHO mannose-6-phosphate glycoproteome. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:46:15.475.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD010333 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | TOMISLAV CAVAL |
SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Phospho; Oxidation; Carbamidomethyl |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-06 05:56:32 | ID requested | |
1 | 2018-12-18 10:43:48 | announced | |
⏵ 2 | 2024-10-22 04:46:16 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.6019/PXD010333; |
10.1074/mcp.ra118.000967; |
Č, aval T, Zhu J, Tian W, Remmelzwaal S, Yang Z, Clausen H, Heck AJR, Targeted Analysis of Lysosomal Directed Proteins and Their Sites of Mannose-6-phosphate Modification. Mol Cell Proteomics, 18(1):16-27(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: IMAC, Hela, M6P, glycopeptide,CHO |
Contact List
Albert J R Heck |
contact affiliation | Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Science4Life, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands |
contact email | A.J.R.Heck@uu.nl |
lab head | |
TOMISLAV CAVAL |
contact affiliation | Utrecht University |
contact email | t.caval@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010333
- Label: PRIDE project
- Name: Analysis of intact mannose-6-phosphate glycopeptides