PXD010329

PXD010329 is an original dataset announced via ProteomeXchange.

Title	Inosine induces context-dependent recoding and translational stalling
Description	To test how inosine in a codon is translated, we synthesized short reporter-transcripts coding for an N-terminal Flag-tag and a test-peptide containing inosine in one defined codon. All codons containing inosines in at least one position were tested. Codons that would lead to ambiguous translation products were omitted. The transcripts were generated using in vitro transcription with ITP instead of GTP and subsequently in vitro translated using rabbit reticulocyte lysate and resulting peptides were analyzed by LC-MS/MS. The templates for in vitro transcription were generated using linearized DNA plasmids.
HostingRepository	PRIDE
AnnounceDate	2018-11-06
AnnouncementXML	Submission_2018-12-06_02:33:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Konstantin Licht
SpeciesList	scientific name: synthetic construct; NCBI TaxID: 32630;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-06 02:23:12	ID requested
1	2018-11-06 02:23:23	announced
⏵ 2	2018-12-06 02:33:02	announced	Updated publication reference for PubMed record(s): 30462291.

Licht K, Hartl M, Amman F, Anrather D, Janisiw MP, Jantsch MF, Inosine induces context-dependent recoding and translational stalling. Nucleic Acids Res, 47(1):3-14(2019) [pubmed]

curator keyword: Biological

submitter keyword: ADAR, A-to-I editing, mRNA-translation, epitranscriptome, RNA modification, LC-MSMS

Michael F. Jantsch
contact affiliation	Department of Cell- and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Austria
contact email	Michael.Jantsch@meduniwien.ac.at
lab head
Konstantin Licht
contact affiliation	Medical University of Vienna
contact email	konstantin.licht@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010329

PRIDE project URI

• PRIDE
  1. PXD010329
     1. Label: PRIDE project
     2. Name: Inosine induces context-dependent recoding and translational stalling