PXD010327

PXD010327 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Retention Order Reversal of Phosphorylated and Unphosphorylated Peptides in Reversed-Phase LC/MS
Description	Protein phosphorylation is one of the most ubiquitous post-translational modifications in human, and trypsin-digested phosphorylated peptides have been analyzed by reversed phase LC/MS using C18-silica columns under the acidic conditions to profile human phosphoproteomes. Here, we reported that phosphopeptides generally retain stronger than their unphosphorylated counterparts when C18-silica columns are used with acetic acid or formic acid as an ion-pairing reagent, whereas the retention order is reversed when less hydrophobic stationary phases such as C4-silica columns are used or more acidic and hydrophobic ion-pairing reagents such as trifluoroacetic acid are used with C18-silica columns. These phenomena could be explained by smaller S-values of phosphopeptides in linear solvation strength theory, based on the reduced net charge caused by intramolecular interaction between phosphate and basic groups.
HostingRepository	jPOST
AnnounceDate	2018-11-11
AnnouncementXML	Submission_2022-09-18_03:05:16.979.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010327
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kosuke Ogata
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; O4'-phospho-L-tyrosine; O-phospho-L-threonine; O-phospho-L-serine; L-methionine sulfoxide
Instrument	TripleTOF 5600; Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-06 01:31:17	ID requested
1	2018-11-11 05:14:32	announced
2	2021-02-04 17:28:00	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 03:05:17	announced	2022-09-18: Updated FTP location.

Publication List

Ogata K, Krokhin OV, Ishihama Y, Retention Order Reversal of Phosphorylated and Unphosphorylated Peptides in Reversed-Phase LC/MS. Anal Sci, 34(9):1037-1041(2018) [pubmed]

Ogata K, Krokhin OV, Ishihama Y, Retention Order Reversal of Phosphorylated and Unphosphorylated Peptides in Reversed-Phase LC/MS. Anal Sci, 34(9):1037-1041(2018) [pubmed]

Keyword List

submitter keyword: phosphopeptides, retention order reversal, linear solvation strength theory, reversed-phase LC, ion-pairing

submitter keyword: phosphopeptides, retention order reversal, linear solvation strength theory, reversed-phase LC, ion-pairing

Contact List

Yasushi Ishihama
lab head
Kosuke Ogata
contact affiliation	Kyoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000449/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000449/