PXD010324

PXD010324 is an original dataset announced via ProteomeXchange.

Title	Identification of Arabidopsis mitochondrial ribosome proteome
Description	In order to identify the comprehensive composition of Arabidopsis mitochondrial ribosome proteins, a strategy based on complementary approaches was used. Classical biochemical purification of ribosomes was combined with the immunoprecipitation of mitoribosomes using a specific Arabidopsis mitoribosome protein as a bait and quantitative proteomics. The immuno-purifications (IP) of the mitoribosome were performed on purified mitochondria from rPPR1-HA plant line (flowers as starting material). IPs were performed performed in different conditions (i.e. with 100, 400 or 600 mM KCl at the IP washing step or 800mM all along the IP). For each condition, experiments were performed in triplicates and IP proteins were identified by quantitative nano LC-ESI-MS/MS. Classic biochemical purification of mitochondrial monosomes (i.e. free ribosomes) was also performed. Starting from purified wild-type Arabidopsis mitochondria (cell culture as starting material), ribosomes were separated on high resolution 10-30% continuous sucrose gradients and mitoribosome containing fractions were analyzed by quantitative nano LC-ESI-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2018-12-14
AnnouncementXML	Submission_2018-12-14_03:15:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-06 01:16:31	ID requested
⏵ 1	2018-12-14 03:15:21	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Arabidopsis, mitochondria, ribosome, nanoLC-ESI-MS/MS

Florent Waltz
contact affiliation	Institut de biologie de moléculaire des plantes, UPR2357 du CNRS, Université de Strasbourg, 12 rue du général Zimmer, F-67084 Strasbourg, France
contact email	waltz.florent@gmail.com
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD010324

PRIDE project URI

• PRIDE
  1. PXD010324
     1. Label: PRIDE project
     2. Name: Identification of Arabidopsis mitochondrial ribosome proteome