PXD010315

PXD010315 is an original dataset announced via ProteomeXchange.

Title	Improving fractionation of human milk proteins through calcium phosphate co-precipitation and their rapid characterization by capillary electrophoresis
Description	This work describes a simple sample pretreatment method for the fractionation of human milk proteins (into their two main groups: whey and caseins) prior to their analysis. The protein extraction protocol is based on the addition of calcium phosphate to non-adjusted pH human milk. The combination of calcium ions with phosphate results in an effective co-precipitation of caseins. To assess the suitability of this fractionation protocol, the protein extracts were analyzed by SDS-PAGE, LC-MS/MS and CE analysis. The results evidence a significant decrease in contamination of casein fraction with whey proteins and vice versa compared with the conventional isoelectric precipitation of caseins. In addition, CE fraction collection coupled to LC-MS/MS (off-line coupling) has been successfully applied to the identification of minor proteins in this complex matrix. The methodology presented here constitutes a promising tool to enlarge the knowledge of human milk proteome.
HostingRepository	PRIDE
AnnounceDate	2018-08-31
AnnouncementXML	Submission_2018-08-31_06:33:24.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010315
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-05 02:31:31	ID requested
⏵ 1	2018-08-31 06:33:25	announced

Ten-Dom, é, nech I, Sim, ó, -Alfonso EF, Herrero-Mart, í, nez JM, Improving Fractionation of Human Milk Proteins through Calcium Phosphate Coprecipitation and Their Rapid Characterization by Capillary Electrophoresis. J Proteome Res, 17(10):3557-3564(2018) [pubmed]

curator keyword: Technical

submitter keyword: calcium phosphate fractionation

human milk whey proteins

human milk caseins

capillary electrophoresis

fraction collection

Dr. José Manuel Herrero-Martínez
contact affiliation	Department of Analytical Chemistry, University of ValenciCC. Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain
contact email	jmherrer@uv.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010315
     1. Label: PRIDE project
     2. Name: Improving fractionation of human milk proteins through calcium phosphate co-precipitation and their rapid characterization by capillary electrophoresis