PXD010298

PXD010298 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics analysis of saliva and salivary gland tissue from the common octopus Octopus vulgaris
Description	The saliva of the common octopus (Octopus vulgaris) has been the subject of biochemical study for over a century. A combination of bioassays, behavioural studies and molecular analysis on O. vulgaris and related species suggests that it should contain a mixture of highly potent neurotoxins and degradative proteins. However, a lack of genomic and transcriptomic data has meant that the amino acid sequences of these proteins remain almost entirely unknown. To address this, we assembled the salivary gland transcriptome of O. vulgaris and combined it with high resolution mass spectrometry data from the posterior and anterior salivary glands of two adults, the posterior salivary glands of six paralarvae and the saliva from a single adult. We identified a total of 2810 protein groups from across this range of salivary tissues and age classes, including 84 with homology to known venom protein families. Additionally, we found 21 short secreted cysteine rich protein groups of which 12 were specific to cephalopods. By combining protein expression data with phylogenetic analysis we demonstrate that serine proteases expanded dramatically within the cephalopod lineage and that cephalopod specific proteins are strongly associated with the salivary apparatus.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:23:10.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ira Cooke
SpeciesList	scientific name: Octopus vulgaris; NCBI TaxID: 6645;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-03 05:55:49	ID requested
1	2018-09-24 03:32:03	announced
2	2019-02-12 08:23:37	announced	Updated publication reference for PubMed record(s): 30220204.
⏵ 3	2024-10-22 04:23:18	announced	2024-10-22: Updated project metadata.

Fingerhut LCHW, Strugnell JM, Faou P, Labiaga Á R, Zhang J, Cooke IR, Shotgun Proteomics Analysis of Saliva and Salivary Gland Tissue from the Common Octopus Octopus vulgaris. J Proteome Res, 17(11):3866-3876(2018) [pubmed]

10.1021/acs.jproteome.8b00525;

curator keyword: Biological

submitter keyword: LC-MSMS,Octopus vulgaris

Jan Strugnell
contact affiliation	James Cook University
contact email	jan.strugnell@jcu.edu.au
lab head
Ira Cooke
contact affiliation	James Cook University
contact email	ira.cooke@jcu.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD010298

PRIDE project URI

• PRIDE
  1. PXD010298
     1. Label: PRIDE project
     2. Name: Shotgun proteomics analysis of saliva and salivary gland tissue from the common octopus Octopus vulgaris