PXD010290

PXD010290 is an original dataset announced via ProteomeXchange.

Title	Deciphering the roles of N-glycans on collagen-platelet interactions
Description	Collagen is a potent agonist for platelet activation, presenting itself as a key contributor to coagulation via interactions with platelet glycoproteins. The fine-details dictating platelet-collagen interactions are poorly understood. In particular, glycosylation could be a key determinant in the platelet-collagen interaction. Here we report an affinity purification coupled to mass spectrometry-based approach to elucidate the function of N-glycans in dictating platelet-collagen interactions. By integrative proteomic and glycoproteomic analysis of collagen-platelet interactive proteins with N-glycan manipulation, we demonstrate that the interaction of platelet adhesive receptors with collagen are highly N-glycan regulated, with glycans on many receptors playing positive roles on collagen binding, with glycans on other platelet glycoproteins exhibiting inhibitory roles on the binding to collagen. Our results significantly enhance our understanding of the details of glycans influencing the platelet-collagen interaction.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:19.818.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Toonstra
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-03 03:23:03	ID requested
1	2019-06-14 01:07:07	announced
⏵ 2	2024-10-22 04:46:28	announced	2024-10-22: Updated project metadata.

Toonstra C, Hu Y, Zhang H, Deciphering the Roles of N-Glycans on Collagen-Platelet Interactions. J Proteome Res, 18(6):2467-2477(2019) [pubmed]

10.1021/acs.jproteome.9b00003;

curator keyword: Biological

submitter keyword: Collagen, Protein Interactions, Glycans,Platelets

Hui Zhang
contact affiliation	Department of Pathology, Johns Hopkins School of Medicine, United States of America
contact email	huizhang@jhu.edu
lab head
Christian Toonstra
contact affiliation	University of Minnesota
contact email	toonstrac@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010290
     1. Label: PRIDE project
     2. Name: Deciphering the roles of N-glycans on collagen-platelet interactions