PXD010269

PXD010269 is an original dataset announced via ProteomeXchange.

Title	Recombinant extracellular vesicles as a biological reference material
Description	Recent years have seen a tremendous increase in the study of extracellular vesicles (EV) geared towards biological understanding, diagnostics and therapy. The interpretation of EV data however remains challenging owing to the complexity of biofluids and the technical variability that are introduced during sample preparation. To understand and mitigate these errors we present the use of traceable recombinant EV (rEV) that mimic endogenous EV both physically and biochemically. The informed use of rEV ensures standardized measurements in various research and biomedical applications. In this dataset we performed mass spectrometry-based proteomics analyses to compare the protein content of endogenous EVs (eEV) and traceable recombinant EV (rEV). The analysis showed that both types of EVs are very similar and that more than 90% of proteins are equally abundant in rEV and eEV isolated from HEK293T cells.
HostingRepository	PRIDE
AnnounceDate	2019-07-11
AnnouncementXML	Submission_2019-08-12_07:32:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evy Timmerman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-29 02:03:25	ID requested
1	2019-07-10 18:54:05	announced
⏵ 2	2019-08-12 07:32:42	announced	Updated publication reference for PubMed record(s): 31337761.

Geeurickx E, Tulkens J, Dhondt B, Van Deun J, Lippens L, Vergauwen G, Heyrman E, De Sutter D, Gevaert K, Impens F, Miinalainen I, Van Bockstal PJ, De Beer T, Wauben MHM, Nolte-'t-Hoen ENM, Bloch K, Swinnen JV, van der Pol E, Nieuwland R, Braems G, Callewaert N, Mestdagh P, Vandesompele J, Denys H, Eyckerman S, De Wever O, Hendrix A, The generation and use of recombinant extracellular vesicles as biological reference material. Nat Commun, 10(1):3288(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: proteomics, mass spectrometry, VLPs, rEVs, HEK293T cells

An Hendrix
contact affiliation	Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research,Ghent University, Ghent, Belgium
contact email	an.hendrix@ugent.be
lab head
Evy Timmerman
contact affiliation	Ghent University - VIB
contact email	evy.timmerman@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD010269

PRIDE project URI

• PRIDE
  1. PXD010269
     1. Label: PRIDE project
     2. Name: Recombinant extracellular vesicles as a biological reference material