PXD010264

PXD010264 is an original dataset announced via ProteomeXchange.

Title	Comparison of the proteome-wide alkylation signatures of antimalarial peroxides: ozonides vs. artemisinin
Description	Antimalarial peroxides such as the phytochemical artemisinin or the synthetic artefenomel are activated by reductive cleavage of the peroxide bond. This happens inside the malaria parasite, presumably in the food vacuole with ferrous heme as the electron donor. The generated carbon-centered radicals will then alkylate heme itself as well as proteins. Here we determine the proteinaceous alkylation signatures of artemisinin and synthetic ozonides by chemical proteomics in Plasmodium falciparum, using alkyne probes to identify target proteins by click chemistry, affinity purification and mass spectrometry-based proteomics. Two forms of negative controls were included: the non-clickable parent peroxides, as well as clickable but non-peroxidic derivatives. Using this approach and stringent purification procedures, we identified 25 P. falciparum proteins that were alkylated by the antimalarial peroxides in a peroxide-dependent manner and at physiological concentration (100 ng/ml); higher exposure (1000 ng/ml) added another 19 proteins to the target lists. Gene ontology term enrichment was performed to analyze the differences between the alkylation signatures of ozonide- and artemisinin-based alkyne probes. This identified overrepresentation of unfolded protein binding, in particular the chaperonin-containing T-complex (TRiC), as the main difference between ozonides and artemisinin, the very mechanism that is involved in artemisinin resistance. Thus differences in the alkylation signatures may account for the lack of cross-resistance between artemisinin and artefenomel.
HostingRepository	PRIDE
AnnounceDate	2022-02-24
AnnouncementXML	Submission_2022-02-24_04:23:05.950.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010264
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Plasmodium falciparum NF135/5.C10; NCBI TaxID: 1036726;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-28 23:11:40	ID requested
⏵ 1	2022-02-24 04:23:06	announced

Jourdan J, Walz A, Matile H, Schmidt A, Wu J, Wang X, Dong Y, Vennerstrom JL, Schmidt RS, Wittlin S, M, ä, ser P, Stochastic Protein Alkylation by Antimalarial Peroxides. ACS Infect Dis, 5(12):2067-2075(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Malaria, peroxides, label-free quantification

Alexander Schmidt
contact affiliation	Proteomics Care Facilty, Biozentrum, University of Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010264
     1. Label: PRIDE project
     2. Name: Comparison of the proteome-wide alkylation signatures of antimalarial peroxides: ozonides vs. artemisinin