PXD010250

PXD010250 is an original dataset announced via ProteomeXchange.

Title	Tolerant Coxiella burnetii survives doxycycline stress: Proteomics reveals crucial mechanisms
Description	Coxiella burnetii is an intracellular pathogen that causes Q fever, a zoonotic disease found worldwide. In humans, the bacterium is responsible for an acute and chronic disease. It is transmitted to humans by the inhalation of aerosols or contaminated dust containing bacteria from infected livestock. Recently C. burnetii re-emerged in Europe making considerable economic impact in farming industry and even causing multiple human cases. Antibiotic resistance represents a global public health problem; moreover, there are few reports about doxycycline resistance in C. burnetii. Understanding drug application consequences in tolerant bacterial strains is indispensable for the effective therapy. Herein, we report changes in proteome of C. burnetii challenged by antibiotic stress, highlighting polypeptides likely involved in the detoxification of doxycycline. These data were corroborated by gene expression analysis. Based on the identities of differentially abundant proteins we suggest that homeostasis of bacterial surface, oxidative stress defense and balanced carbon metabolism are key processes to survive doxycycline treatment.
HostingRepository	PRIDE
AnnounceDate	2019-08-14
AnnouncementXML	Submission_2019-08-14_04:27:04.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010250
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Coxiella burnetii RSA 331; NCBI TaxID: 360115;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-27 05:56:20	ID requested
⏵ 1	2019-08-14 04:27:06	announced

Zu, ñ, iga-Navarrete F, Flores-Ramirez G, Danchenko M, Benada O, Skriba A, Skultety L, Proteomic analysis revealed the survival strategy of Coxiella burnetii to doxycycline exposure. J Proteomics, 208():103479(2019) [pubmed]

curator keyword: Biological

submitter keyword: Q fever, antibiotic, two-dimensional gel electrophoresis, axenic medium, cell envelope homeostasis, enzymatic antioxidants

Gabriela Flores-Ramirez
contact affiliation	Biomedical Research Center, Slovak Academy of Sciences
contact email	virugafl@savba.sk
lab head
Maksym Danchenko
contact affiliation	Institute of Virology, Biomedical Research Center
contact email	maxymdan@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010250
     1. Label: PRIDE project
     2. Name: Tolerant Coxiella burnetii survives doxycycline stress: Proteomics reveals crucial mechanisms