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PXD010247

PXD010247 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome profiling of extracellular vesicles from neurosurgical aspirates identifies CCT6A as a potential glioblastoma biomarker with prognostic significance
DescriptionGlioblastoma, WHO-grade IV glioma, carries a dismal prognosis owing to its infiltrative growth rates and limited treatment options. Glioblastoma-derived extracellular vesicles (EVs; 30-1000nm membranous particles) influence the microenvironment to mediate tumour aggressiveness and carry oncogenic cargo across the blood-brain-barrier into the circulation. As such, EVs are biomarker reservoirs with enormous potential for assessing glioblastoma tumours in situ. Neurosurgical aspirates are rich sources of EVs, isolated directly from glioma microenvironments. Quantitative LC-MS/MS compared EV proteomes enriched from glioblastoma (n=15) and glioma grade II-III (n=7) aspirates and identified 298 differentially-abundant proteins (p-value<0.00496). Along with previously reported glioblastoma-associated biomarkers, levels of all eight subunits of the key molecular chaperone, T-complex protein 1 Ring complex (TRiC), were higher in glioblastoma-EVs, including CCT2, CCT3, CCT5, CCT6A, CCT7 and TCP1 (p<0.00496). Analogous increases in TRiC transcript levels and DNA copy numbers were detected in silico; CCT6A had the greatest induction of expression and amplification in glioblastoma and showed a negative association with survival (p=0.006). CCT6A is co-localised with EGFR at 7p11.2, with a strong tendency for co-amplification (p<0.001). Immunohistochemistry corroborated the CCT6A proteomics measurements and indicated a potential link between EGFR and CCT6A tissue expressions. Putative EV-biomarkers described here should be further assessed in peripheral blood.
HostingRepositoryPRIDE
AnnounceDate2022-03-01
AnnouncementXMLSubmission_2022-03-01_15:04:30.177.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD010247
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSusannah Hallal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-06-27 05:06:37ID requested
12022-03-01 15:04:31announced
Publication List
Hallal S, Russell BP, Wei H, Lee MYT, Toon CW, Sy J, Shivalingam B, Buckland ME, Kaufman KL, Extracellular Vesicles from Neurosurgical Aspirates Identifies Chaperonin Containing TCP1 Subunit 6A as a Potential Glioblastoma Biomarker with Prognostic Significance. Proteomics, 19(1-2):e1800157(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Human, Glioblastoma, Glioma, Brain, Tumour, LC-MS/MS, Extracellular Vesicles, Exosomes, Biomarker, CCT6A
Contact List
Kimberley Louise Kaufman
contact affiliationBrainstorm Brain Cancer Research, Brain and Mind Centre, University of Sydney, NSW, Australia School of Life and Environmental Science, University of Sydney, New South Wales, Australia
contact emailkim.kaufman@sydney.edu.au
lab head
Susannah Hallal
contact affiliationThe University of Sydney
contact emailsusannah.hallal@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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