PXD010244

PXD010244 is an original dataset announced via ProteomeXchange.

Title	Decreased antibiotic susceptibility driven by global remodeling of the Klebsiella pneumoniae proteome
Description	Bacteria can circumvent the effect of antibiotics by transitioning to a poorly understood physiological state that does not involve conventional genetic elements of resistance. Here we examine antibiotic susceptibility with a Class A β-lactamase+ invasive strain of Klebsiella pneumoniae that was isolated from a lethal outbreak within laboratory colonies of Chlorocebus aethiops sabaeus monkeys. Bacterial responses to the ribosomal synthesis inhibitors streptomycin and doxycycline resulted in distinct proteomic adjustments that facilitated decreased susceptibility to each antibiotic. Drug-specific changes to proteomes included proteins for receptor-mediated membrane transport and sugar utilization, central metabolism, and capsule production, while mechanisms common to both antibiotics included elevated scavenging of reactive oxygen species and turnover of misfolded proteins. Resistance to combined antibiotics presented integrated adjustments to protein levels as well as unique drug-specific proteomic features. Our results demonstrate that dampening of Klebsiella pneumoniae susceptibility involves global remodeling of the bacterial proteome to counter the effects of antibiotics and stabilize growth.
HostingRepository	PRIDE
AnnounceDate	2019-03-14
AnnouncementXML	Submission_2019-03-14_02:35:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moo-Jin Suh
SpeciesList	scientific name: Klebsiella pneumoniae; NCBI TaxID: 573;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-27 03:56:03	ID requested
⏵ 1	2019-03-14 02:35:35	announced

Keasey SL, Suh MJ, Das S, Blancett CD, Zeng X, Andresson T, Sun MG, Ulrich RG, Proteome. Mol Cell Proteomics, 18(4):657-668(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Klebsiella pneumoniae proteome, Antibiotics

Suh, Moo-Jin
contact affiliation	US Army Medical Research Institute of Infectiouos Diseases, Frederick, MD
contact email	jinisuh@gmail.com
lab head
Moo-Jin Suh
contact affiliation	USAMRIID
contact email	jinisuh@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010244
     1. Label: PRIDE project
     2. Name: Decreased antibiotic susceptibility driven by global remodeling of the Klebsiella pneumoniae proteome