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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-03-14
  • AnnouncementXML: Submission_2019-03-14_02:35:34.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Moo-Jin Suh
  • Title: Decreased antibiotic susceptibility driven by global remodeling of the Klebsiella pneumoniae proteome
  • Description: Bacteria can circumvent the effect of antibiotics by transitioning to a poorly understood physiological state that does not involve conventional genetic elements of resistance. Here we examine antibiotic susceptibility with a Class A β-lactamase+ invasive strain of Klebsiella pneumoniae that was isolated from a lethal outbreak within laboratory colonies of Chlorocebus aethiops sabaeus monkeys. Bacterial responses to the ribosomal synthesis inhibitors streptomycin and doxycycline resulted in distinct proteomic adjustments that facilitated decreased susceptibility to each antibiotic. Drug-specific changes to proteomes included proteins for receptor-mediated membrane transport and sugar utilization, central metabolism, and capsule production, while mechanisms common to both antibiotics included elevated scavenging of reactive oxygen species and turnover of misfolded proteins. Resistance to combined antibiotics presented integrated adjustments to protein levels as well as unique drug-specific proteomic features. Our results demonstrate that dampening of Klebsiella pneumoniae susceptibility involves global remodeling of the bacterial proteome to counter the effects of antibiotics and stabilize growth.
  • SpeciesList: scientific name: Klebsiella pneumoniae; NCBI TaxID: 573;
  • ModificationList: iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-06-27 03:56:03ID requested
12019-03-14 02:35:35announced

Publication List

  1. Keasey SL, Suh MJ, Das S, Blancett CD, Zeng X, Andresson T, Sun MG, Ulrich RG, Proteome. Mol Cell Proteomics, 18(4):657-668(2019) [pubmed]

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: Klebsiella pneumoniae proteome, Antibiotics

Contact List

    Suh, Moo-Jin
    • contact affiliation: US Army Medical Research Institute of Infectiouos Diseases, Frederick, MD
    • contact email: jinisuh@gmail.com
    • lab head:
    Moo-Jin Suh
    • contact affiliation: USAMRIID
    • contact email: jinisuh@gmail.com
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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