PXD010238

PXD010238 is an original dataset announced via ProteomeXchange.

Title	Analysis of Erysiphe pisi proteome
Description	Erysiphe pisi causes powdery mildew disease in garden pea. It is a biotrophic ascomycete member necessitating a living host for its survival. An attempt to identify the global proteome of E. pisi pathogen is made using a sensitive and reliable nano LC-MS/MS approach. The protein profiling of two isolates of E. pisi; Ep01 and Ep02 varying for virulence upon testing on a commercial cultivar, Arkel led to the detection of a total of 211 and 214 distinct proteins in Ep01 and Ep03 isolates respectively. In addition, a total of 203 and 207proteins from Ep01 and Ep03 isolates respectively were found to be hypothetical or proteins with not yet predicted functions based on GO (biological process). The protein accessions detected in these isolates were categorized into functional protein classes with some of the identified proteins reported to be involved in pathogenesis or virulence. The proteins belonging to the functional classes like stress related, signal transduction and secondary metabolite formation might be involved in virulence and pathogenesis. The proteome proposed in this study would serve as a reference proteome to facilitate the understanding of the functional aspects of an obligate biotrophic fungal pathogen.
HostingRepository	PRIDE
AnnounceDate	2019-04-02
AnnouncementXML	Submission_2019-04-02_00:01:28.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010238
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ragiba Makandar
SpeciesList	scientific name: Erysiphe pisi; NCBI TaxID: 36044;
ModificationList	Carboxymethyl; Glu->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	LTQ Orbitap Discovery

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-27 01:17:24	ID requested
⏵ 1	2019-04-02 00:01:29	announced

Bheri M, M Bhosle S, Makandar R, Shotgun proteomics provides an insight into pathogenesis-related proteins using anamorphic stage of the biotroph, Erysiphe pisi pathogen of garden pea. Microbiol Res, 222():25-34(2019) [pubmed]

curator keyword: Biological

submitter keyword: Erysiphe pisi, proteome, nano LC-MS/MS, powdery mildew, garden pea

Prof. Ragiba Makandar
contact affiliation	Department of Plant Sciences School of Life Sciences University of Hyderabad Hyderabad-500046, India.
contact email	ragibamakandar@gmail.com
lab head
Ragiba Makandar
contact affiliation	University of Hyderabad
contact email	ragibamakandar@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010238
     1. Label: PRIDE project
     2. Name: Analysis of Erysiphe pisi proteome