PXD010227

PXD010227 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of mulberry in response to drought stress
Description	Mulberry (Morus alba) is a fast-growing perennial woody plant with a long cultivation history in China and a distribution throughout the country. To date, the molecular mechanism of mulberry response to abiotic stress is little known. In the present study, proteomics data were collected from the leaves of the mulberry cultivar Neo-Ichinose (Japan) under drought stress and analyzed. In total, 2871 proteins were quantified, among which 267 proteins were differentially expressed. A Gene Ontology enrichment analysis indicated that the molecular functions of the up-regulated proteins were mainly related to glutathione peroxidase activity, hydrolase activity, oxidoreductase activity, peroxidase activity and antioxidant activity. Protein domain enrichment analysis showed that the most important protein domains were associated with glutathione peroxidase, glycoside hydrolase, and chaperones. Additionally, a functional enrichment analysis demonstrated that the main metabolic pathways of mulberry seedlings in response to drought stress were arachidonic acid metabolism and glutathione metabolism, which were up-regulated, and chlorophyll and porphyrin metabolism and glycerol metabolism, which were down-regulated. Additionally, many proteins related to osmotic adjustment and stress signal transduction were evoked by drought. Taken together, these results suggested that redox control and osmotic adjustment mediated by multiple signaling pathways dominated the drought response of mulberry and that antioxidant proteins and enzymes, especially glutathione peroxidase, played vital roles in the redox rebalance of mulberry under stress.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:09:26.573.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	suzhen huang
SpeciesList	scientific name: Morus alba; NCBI TaxID: 3498;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-26 05:19:30	ID requested
1	2022-10-13 03:08:19	announced
⏵ 2	2023-11-14 07:09:27	announced	2023-11-14: Updated project metadata.

Zhang M, Li W, Li S, Gao J, Gan T, Li Q, Bao L, Jiao F, Su C, Qian Y, Quantitative Proteomics and Functional Characterization Reveal That Glutathione Peroxidases Act as Important Antioxidant Regulators in Mulberry Response to Drought Stress. Plants (Basel), 11(18):(2022) [pubmed]

curator keyword: Biological

submitter keyword: drought stress

proteome

iTRAQ

Yong Hua Qian
contact affiliation	College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China
contact email	qyh@nwsuaf.edu.cn
lab head
suzhen huang
contact affiliation	PTM
contact email	13920817975@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD010227

PRIDE project URI

• PRIDE
  1. PXD010227
     1. Label: PRIDE project
     2. Name: iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of mulberry in response to drought stress