PXD010210

PXD010210 is an original dataset announced via ProteomeXchange.

Title	DIA+: A novel data-independent acquisition method combines multiple precursor charges to boost peptide signal.
Description	Data-independent acquisition (DIA) methods aim to expand the benefits of low-throughput targeted proteomics to proteome-wide analyses. These methods rely on the use of several broadband isolation windows that select and fragment all peptide ions within a cycle. Isolation windows differ in that (I) they exhibit different widths, (ii) they are acquired either sequentially or in a non-consecutive order, and (iii) they are either juxtaposed or overlapped. Here we present DIA+, a novel DIA multiplexing scheme with isolation windows that combine signals from identical peptides with different charges. DIA+ is based on the co-isolation of charge +2 and +3 precursor ions from identical peptide sequences to combine their signal and therefore, increase the number of MS2 peptide fragment ions detected. DIA+ combines three non-consecutive m/z ranges, based on the mass difference between charge +2 and +3 peptides, into a composite 24 Da isolation window. Forty of these combined 24 Da windows cover a range of 400-1350 m/z. The performance of DIA+ was compared with other reference methods in the field, and with a DIA+ control method in which each 24 Da isolation window results from the combination of three consecutive m/z ranges. The combination of improved signal-to-noise and sequence coverage increases the confidence on peptide identification and results in a significant increase in the number of identified peptides and proteins.
HostingRepository	PRIDE
AnnounceDate	2018-10-11
AnnouncementXML	Submission_2018-10-11_06:15:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduard Sabidó
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-25 02:16:16	ID requested
⏵ 1	2018-10-11 06:15:19	announced

Borr, à, s E, Sabid, ó E, DIA+: A Data-Independent Acquisition Method Combining Multiple Precursor Charges to Improve Peptide Signal. Anal Chem, 90(21):12339-12341(2018) [pubmed]

curator keyword: Technical

submitter keyword: DIA, Hela

Eduard Sabidó
contact affiliation	Proteomics Unit Centre for Genomic Regulation University Pompeu Fabra Dr Aiguader 88, 08003 Barcelona, Spain
contact email	eduard.sabido@crg.cat
lab head
Eduard Sabidó
contact affiliation	Centre de Regulació Genòmica
contact email	eduard.sabido@crg.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010210

PRIDE project URI

• PRIDE
  1. PXD010210
     1. Label: PRIDE project
     2. Name: DIA+: A novel data-independent acquisition method combines multiple precursor charges to boost peptide signal.