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PXD010210

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-10-11
  • AnnouncementXML: Submission_2018-10-11_06:15:18.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Eduard Sabidˇ
  • Title: DIA+: A novel data-independent acquisition method combines multiple precursor charges to boost peptide signal.
  • Description: Data-independent acquisition (DIA) methods aim to expand the benefits of low-throughput targeted proteomics to proteome-wide analyses. These methods rely on the use of several broadband isolation windows that select and fragment all peptide ions within a cycle. Isolation windows differ in that (I) they exhibit different widths, (ii) they are acquired either sequentially or in a non-consecutive order, and (iii) they are either juxtaposed or overlapped. Here we present DIA+, a novel DIA multiplexing scheme with isolation windows that combine signals from identical peptides with different charges. DIA+ is based on the co-isolation of charge +2 and +3 precursor ions from identical peptide sequences to combine their signal and therefore, increase the number of MS2 peptide fragment ions detected. DIA+ combines three non-consecutive m/z ranges, based on the mass difference between charge +2 and +3 peptides, into a composite 24 Da isolation window. Forty of these combined 24 Da windows cover a range of 400-1350 m/z. The performance of DIA+ was compared with other reference methods in the field, and with a DIA+ control method in which each 24 Da isolation window results from the combination of three consecutive m/z ranges. The combination of improved signal-to-noise and sequence coverage increases the confidence on peptide identification and results in a significant increase in the number of identified peptides and proteins.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: Orbitrap Fusion Lumos

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-06-25 02:16:16ID requested
12018-10-11 06:15:19announced

Publication List

  1. BorrÓs E, Sabidˇ E, DIA+: A Data-Independent Acquisition Method Combining Multiple Precursor Charges to Improve Peptide Signal. Anal Chem, 90(21):12339-12341(2018) [pubmed]

Keyword List

  1. curator keyword: Technical
  2. submitter keyword: DIA, Hela

Contact List

    Eduard Sabidˇ
    • contact affiliation: Proteomics Unit Centre for Genomic Regulation University Pompeu Fabra Dr Aiguader 88, 08003 Barcelona, Spain
    • contact email: eduard.sabido@crg.cat
    • lab head:
    Eduard Sabidˇ
    • contact affiliation: Centre de Regulaciˇ Gen˛mica
    • contact email: eduard.sabido@crg.cat
    • dataset submitter:

Full Dataset Link List

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