PXD010209

PXD010209 is an original dataset announced via ProteomeXchange.

Title	Rat Liver HFD vs Chow, HFD vs PKCepsilon ASO
Description	Insulin resistance drives the development of type 2 diabetes (T2D). In liver, diacylglycerol (DAG) is a key mediator of lipid-induced insulin resistance. DAG activates protein kinase C epsilon (PKCε), which phosphorylates and inhibits the insulin receptor. In rats, a 3-day high fat diet produces hepatic insulin resistance through this mechanism, and knockdown of hepatic PKCε protects against high fat diet-induced hepatic insulin resistance. Here we employ a systems level approach to uncover additional signaling pathways involved in high fat diet-induced hepatic insulin resistance. We used quantitative phosphoproteomics to map global in vivo changes in hepatic protein phosphorylation in chow-fed, high fat-fed, and high fat-fed with PKCε knockdown rats to distinguish the impact of lipid- and PKCε-induced protein phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2018-08-30
AnnouncementXML	Submission_2018-08-30_03:23:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brandon Gassaway
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; deaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-25 02:00:45	ID requested
⏵ 1	2018-08-30 03:23:10	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Rat Liver, Insulin Resistance

Jesse Rinehart
contact affiliation	Cellular & Molecular Physiology, Systems Biology Institute, Yale University
contact email	jesse.rinehart@yale.edu
lab head
Brandon Gassaway
contact affiliation	Yale University
contact email	brandon.gassaway@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010209

PRIDE project URI

• PRIDE
  1. PXD010209
     1. Label: PRIDE project
     2. Name: Rat Liver HFD vs Chow, HFD vs PKCepsilon ASO