PXD010207

PXD010207 is an original dataset announced via ProteomeXchange.

Title	TMEM41B is a novel regulator of autophagy and lipid mobilization
Description	Autophagy maintains cellular homeostasis by targeting damaged organelles, pathogens or misfolded protein aggregates for lysosomal degradation. The autophagic process is initiated by the formation of autophagosomes, which can selectively enclose cargo via autophagy cargo receptors. A machinery of well-characterized autophagy-related proteins orchestrate the biogenesis of autophagosomes, however, the origin of the required membranes is incompletely understood. Here, we applied sensitized pooled CRISPR screens and identified the uncharacterized transmembrane protein TMEM41B as a novel regulator of autophagy. In the absence of TMEM41B, autophagosome biogenesis is stalled, LC3 accumulates at WIPI2- and DFCP1-positive isolation membranes, and lysosomal flux of autophagy cargo receptors and intracellular bacteria is impaired. In addition to defective autophagy, we found that TMEM41B knockout cells display significantly enlarged lipid droplets and reduced mobilization and β-oxidation of fatty acids. TMEM41B localizes to the endoplasmic reticulum (ER) and interacts with SIGMAR1, a chaperone which regulates calcium and lipid transfer at ER contact sites with mitochondria and lipid droplets. Taken together, we propose that TMEM41B is a novel regulator of autophagosome biogenesis and ER lipid mobilization.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:58.578.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Debora Bonenfant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-25 00:08:13	ID requested
1	2018-08-20 03:51:59	announced
2	2018-08-27 03:06:38	announced	Updated publication reference for PubMed record(s): 30126924.
⏵ 3	2024-10-22 04:46:04	announced	2024-10-22: Updated project metadata.

Moretti F, Bergman P, Dodgson S, Marcellin D, Claerr I, Goodwin JM, DeJesus R, Kang Z, Antczak C, Begue D, Bonenfant D, Graff A, Genoud C, Reece-Hoyes JS, Russ C, Yang Z, Hoffman GR, Mueller M, Murphy LO, Xavier RJ, Nyfeler B, TMEM41B is a novel regulator of autophagy and lipid mobilization. EMBO Rep, 19(9):(2018) [pubmed]

10.15252/embr.201845889;

curator keyword: Biological

submitter keyword: autophagy/ contact sites/ CRISPR/ lipid droplets/ TMEM41B

Bonenfant Debora
contact affiliation	Novartis Institutes for BioMedical Research, Novartis Campus, CH-4056 Basel, Switzerland
contact email	debora.bonenfant@novartis.com
lab head
Debora Bonenfant
contact affiliation	Novartis Institutes for BioMedical Research
contact email	debora.bonenfant@novartis.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010207

PRIDE project URI

• PRIDE
  1. PXD010207
     1. Label: PRIDE project
     2. Name: TMEM41B is a novel regulator of autophagy and lipid mobilization