PXD010202

PXD010202 is an original dataset announced via ProteomeXchange.

Title	Identification of PWWP2A interactors using label-free quantitative affinity enrichment mass spectrometry
Description	Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified PWWP2A as a binder for H2A.Z-containing nucleosomes (Pünzeler, S. et al., EMBO J 2017). Here, as part of a larger project trying to characterize PWWP2A, we performed pulldowns using PWWP2A as a bait protein. Label-free quantitative mass spectrometry-based proteomics was used to detect and relatively quantify interaction partners of PWWP2A.
HostingRepository	PRIDE
AnnounceDate	2018-08-30
AnnouncementXML	Submission_2018-10-24_06:55:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-22 08:22:33	ID requested
1	2018-08-30 03:25:11	announced
⏵ 2	2018-10-24 06:56:00	announced	Updated publication reference for PubMed record(s): 30327463.

Link S, Spitzer RMM, Sana M, Torrado M, V, ö, lker-Albert MC, Keilhauer EC, Burgold T, P, ü, nzeler S, Low JKK, Lindstr, ö, m I, Nist A, Regnard C, Stiewe T, Hendrich B, Imhof A, Mann M, Mackay JP, Bartkuhn M, Hake SB, PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nat Commun, 9(1):4300(2018) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Epigenetic Chromatin (B/D-HPP), Human Proteome Project

curator keyword: Biological

submitter keyword: Histone variants, interaction proteomics, AP-MS, AE-MS, label-free quantification, PWWP2A, H2A.Z, MTA1, NuRD

Matthias Mann
contact affiliation	Max Planck Institute of Biochemistry Department Proteomics and Signal Transduction Am Klopferspitz 18 82152 Martinsried, Germany
contact email	mmann@biochem-mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010202

PRIDE project URI

• PRIDE
  1. PXD010202
     1. Label: PRIDE project
     2. Name: Identification of PWWP2A interactors using label-free quantitative affinity enrichment mass spectrometry