PXD010199

PXD010199 is an original dataset announced via ProteomeXchange.

Title	Dyslipidemia Alters HDL Metabolism and Function in NAFLD
Description	Dyslipidemia and inflammation play key roles in the pathogenesis of both nonalcoholic fatty liver disease (NAFLD) and atherosclerosis. NAFLD, particularly its severe form nonalcoholic steatohepatitis (NASH) is associated with increased cardiovascular disease (CVD) risk. HDL (high density lipoprotein- also a CVD risk) are decreased in NAFLD but whether HDL function is abnormal in NAFLD is unknown. Furthermore, it is unknown whether dyslipidemia contributes to reduced HDL function in NAFLD and whether hepatic inflammation further impairs HDL function in patients with NASH. Therefore, the aim of this study was to investigate HDL function and to examine the effect of dyslipidemia and inflammation on HDL metabolism in patients with biopsy-proven simple steatosis (SS) and NASH. RESULTS: Compared to controls, SS and NASH subjects had significantly higher levels of plasma triglyceride, insulin, and were more insulin resistant (HOMA, P<0.05) with no differences in total cholesterol, HDL cholesterol, ApoB100 and ApoAI levels. NAFLD patients had increased production and degradation rates of both HDLc and ApoAI that resulted in their levels remaining stable. The degradation rates also were increased of other HDL proteins, including ApoAII, ApoAIV, vitamin D-binding protein, and complement 3 (all P<0.05). NAFLD patients had increased activities of LCAT and CETP, indicating altered HDL lipidation. NAFLD induced alterations in HDL metabolism were associated with reduced anti-oxidant but increased pro-inflammatory activity of HDL. However, no differences were observed in either HDL function or the kinetics of HDLc and HDL proteins between SS and NASH subjects.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:50.397.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Serguei Ilchenko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized histidine; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-22 05:51:49	ID requested
1	2019-08-12 06:23:05	announced
⏵ 2	2024-10-22 04:46:50	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: SS, nonalcoholic, metabolism,NAFLD, steatosis, Dyslipidemia, hepatic , simple, NASH, inflammation, disease, cardiovascular, steatohepatitis

Takhar Kasumov
contact affiliation	Department of Pharmaceutical Sciences Northeast Ohio Medical University Rootstown, OH, USA
contact email	Tkasumov@neomed.edu
lab head
Serguei Ilchenko
contact affiliation	Neomed
contact email	silchenko@neomed.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010199
     1. Label: PRIDE project
     2. Name: Dyslipidemia Alters HDL Metabolism and Function in NAFLD