PXD010179 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spatiotemporal proteomics uncovers cathepsin-dependent host cell death during bacterial infection |
Description | Immune cells need to swiftly and effectively respond to invading pathogens. This response relies heavily on rapid protein synthesis and accurate cellular targeting to ensure pathogen destruction. In return, pathogens intercept this response so they can survive and proliferate. To gain insight into this dynamic interface, we combined click-chemistry with pulsed stable isotope labelling of amino acids (pSILAC-AHA) and quantified the newly synthesised host proteome during macrophage infection with the model intracellular bacterial pathogen, Salmonella enterica Typhimurium (STm). We monitored newly synthesized proteins across different compartments and during different infection stages, and used available proteomics data in response to LPS to deconvolute the STm-specific response. Within this rich resource, we detect aberrant trafficking of lysosomal proteases to the extracellular space and the nucleus, the latter of which correlates with signatures of cell death. Pharmacological cathepsin inhibition suppressed caspase-11 dependent macrophage cell death, thus demonstrating an active role for cathepsins during STm induced pyroptosis. Our study illustrates the utility of resolving the host proteome dynamics during infection to drive the discovery of novel biological mechanisms at the host-microbe interface. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-24 |
AnnouncementXML | Submission_2020-06-15_02:56:43.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sophia Foehr |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-21 06:09:34 | ID requested | |
1 | 2020-01-24 02:18:31 | announced | |
⏵ 2 | 2020-06-15 02:56:44 | announced | 2020-06-15: Updated publication reference for PubMed record(s): 32514074. |
Publication List
Selkrig J, Li N, Hausmann A, Mangan MSJ, Zietek M, Mateus A, Bobonis J, Sueki A, Imamura H, El Debs B, Sigismondo G, Florea BI, Overkleeft HS, Kopitar-Jerala N, Turk B, Beltrao P, Savitski MM, Latz E, Hardt WD, Krijgsveld J, Typas A, Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection. Nat Microbiol, 5(9):1119-1133(2020) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: pulse SILAC, azidohomoalanine (AHA), salmonella, infection, cathepsin |
Contact List
Jeroen Krijgsveld |
contact affiliation | DKFZ German Cancer Research Center |
contact email | j.krijgsveld@dkfz.de |
lab head | |
Sophia Foehr |
contact affiliation | DKFZ |
contact email | sophia.foehr@dkfz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010179
- Label: PRIDE project
- Name: Spatiotemporal proteomics uncovers cathepsin-dependent host cell death during bacterial infection