PXD010178

PXD010178 is an original dataset announced via ProteomeXchange.

Title	Enhanced validation of antibodies for research applications
Description	There is a need for standardized validation methods for antibody specificity and selectivity. Recently, five alternative validation “pillars" were proposed to explore the specificity of research antibodies using methods with no need for prior knowledge about the protein target. Here, we show that these principles can be used in a streamlined manner for enhanced validation of research antibodies in Western blot applications. More than 6,000 antibodies were validated with at least one of these strategies involving orthogonal methods, genetic knockdown, recombinant expression, independent antibodies and migration capture mass spectrometry analysis. The results show a path forward for efforts to validate antibodies in an application-specific manner suitable for both providers and users.
HostingRepository	PRIDE
AnnounceDate	2018-09-06
AnnouncementXML	Submission_2018-09-06_02:20:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Hober
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics micrOTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-21 06:08:32	ID requested
1	2018-08-21 11:24:04	announced
⏵ 2	2018-09-06 02:20:54	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: LC-MSMS, Western blot, SDS-PAGE, U251, RT4

Mathias Uhlen
contact affiliation	KTH Royal Institute of Technology: Stockholm, Stockholm, Sweden School of Engineering Sciences in Chemistry, Biotechnology and Health
contact email	mathias.uhlen@scilifelab.se
lab head
Andreas Hober
contact affiliation	KTH Royal Institute of Technology
contact email	andreas.hober@scilifelab.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010178

PRIDE project URI

• PRIDE
  1. PXD010178
     1. Label: PRIDE project
     2. Name: Enhanced validation of antibodies for research applications