PXD010178 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Enhanced validation of antibodies for research applications |
Description | There is a need for standardized validation methods for antibody specificity and selectivity. Recently, five alternative validation “pillars" were proposed to explore the specificity of research antibodies using methods with no need for prior knowledge about the protein target. Here, we show that these principles can be used in a streamlined manner for enhanced validation of research antibodies in Western blot applications. More than 6,000 antibodies were validated with at least one of these strategies involving orthogonal methods, genetic knockdown, recombinant expression, independent antibodies and migration capture mass spectrometry analysis. The results show a path forward for efforts to validate antibodies in an application-specific manner suitable for both providers and users. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:45:58.309.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreas Hober |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics micrOTOF series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-21 06:08:32 | ID requested | |
1 | 2018-08-21 11:24:04 | announced | |
2 | 2018-09-06 02:20:54 | announced | Updated project metadata. |
⏵ 3 | 2024-10-22 04:45:58 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: Western blot, SDS-PAGE, U251, RT4,LC-MSMS |
Contact List
Mathias Uhlen |
contact affiliation | KTH Royal Institute of Technology: Stockholm, Stockholm, Sweden School of Engineering Sciences in Chemistry, Biotechnology and Health |
contact email | mathias.uhlen@scilifelab.se |
lab head | |
Andreas Hober |
contact affiliation | KTH Royal Institute of Technology |
contact email | andreas.hober@scilifelab.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010178 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010178
- Label: PRIDE project
- Name: Enhanced validation of antibodies for research applications