PXD010177

PXD010177 is an original dataset announced via ProteomeXchange.

Title	Reconstitution of mammalian Cleavage Factor II
Description	Cleavage factor II (CF II) is a poorly characterized component of the multi-protein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1. The heterodimer is active in partially reconstituted cleavage reactions, whereas hClp1 by itself is not. Pcf11 moderately stimulates the RNA 5' kinase activity of hClp1; the kinase activity is dispensable for RNA cleavage. CF II binds RNA with nanomolar affinity. Binding is mediated mostly by the two zinc fingers in the C-terminal region of hPcf11. RNA is bound without pronounced sequence-specificity, but extended G-rich sequences appear to be preferred. We discuss the possibility that CF II contributes to the recognition of cleavage/polyadenylation substrates through interaction with G-rich far-downstream sequence elements.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-27_03:09:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Tüting
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-21 04:13:01	ID requested
⏵ 1	2020-05-27 03:09:46	announced

Sch, ä, fer P, T, ü, ting C, Sch, ö, nemann L, K, ü, hn U, Treiber T, Treiber N, Ihling C, Graber A, Keller W, Meister G, Sinz A, Wahle E, Reconstitution of mammalian cleavage factor II involved in 3' processing of mRNA precursors. RNA, 24(12):1721-1737(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, RNA processing, DSBU

Elmar Wahle
contact affiliation	Institute for Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg
contact email	ewahle@biochemtech.uni-halle.de
lab head
Christian Tüting
contact affiliation	MLU Halle-Wittenberg
contact email	christian.tueting@biochemtech.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010177
     1. Label: PRIDE project
     2. Name: Reconstitution of mammalian Cleavage Factor II