PXD010171

PXD010171 is an original dataset announced via ProteomeXchange.

Title	Extraction of Entamoeba histolytica membrane proteins using three extraction methods
Description	Entamoeba histolytica membrane proteins are important players in the parasite’s pathogenicity. However, most of the proteins have not been identified. This study reports the membrane proteins extracted using three extractions methods: two commercial kits (ProteoExtract® from Calbiochem and ProteoPrep® from Sigma), and a conventional laboratory method. The resulting membrane fractions (MF) and cytosolic fractions (CF)were analysed using LC-ESI-MS/MS. The proteins identified in at least two out of three biological replicates revealed a total of 490, 492, and 587 MF proteins extracted using the ProteoExtract® kit, ProteoPrep® kit and conventional method, respectively. Meanwhile, 487, 611 and 343 proteins were identified in the CF extracted using the ProteoExtract® kit, ProteoPrep® kit and conventional method, respectively. Analysis of the identified MF and CF proteins extracted by the respective extraction kits suggests that the ProteoPrep® extraction kit was the most selective in separating MF and CF among the three extraction methods.
HostingRepository	PRIDE
AnnounceDate	2018-10-16
AnnouncementXML	Submission_2018-10-15_20:59:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JORIM UJANG
SpeciesList	scientific name: Entamoeba histolytica HM-1:IMSS; NCBI TaxID: 294381;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-20 02:08:45	ID requested
⏵ 1	2018-10-15 20:59:39	announced

Ujang J, Sani AAA, Lim BH, Noordin R, Othman N, Analysis of Entamoeba histolytica Membrane Proteome Using Three Extraction Methods. Proteomics, 18(23):e1700397(2018) [pubmed]

curator keyword: Biological

submitter keyword: Entamoeba histolytica, membrane proteome, membrane protein extraction methods, LC-ESI-MS/MS

Nurulhasanah binti Othman
contact affiliation	Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Penang, Malaysia
contact email	nurulhasanah@usm.my
lab head
JORIM UJANG
contact affiliation	UNIVERSITI SAINS MALAYSIA
contact email	jorim.ujang@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010171
     1. Label: PRIDE project
     2. Name: Extraction of Entamoeba histolytica membrane proteins using three extraction methods