PXD010169

PXD010169 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of the proteolytic events in the secretome of the transformed phenotype of melanocyte-derived cells using Terminal Amine Isotopic Labeling of Substrates
Description	The comprehensive profiling of the repertoire of secreted proteins from cancer cells and/or tissue samples provides information on the signaling events that take place during oncogenesis as well as on the cross-talk between normal and tumoral cells. Moreover, the analysis of post translational modifications in secreted proteins may unravel biological circuits regulated by irreversible modifications such as, for example, proteolytic processing. In this context, we used Terminal Amine Isotopic Labeling of Substrates (TAILS) to perform a system-wide investigation on the N-terminome of the secretomes derived from a paired set of mouse melanocyte cell lines: Melan-a (a normal melanocyte) and Tm1 (its transformed phenotype). TAILS analysis allowed the profiling of co-translational modifications such as acetylated N-termini as well as proteolytic events in both secretomes. Although no significant difference has been found in the proportion of acetylated natural N-termini in both cell line secretomes, when evaluating amino acid identities at the scissile bond in internal peptides it was possible to detect significant differences, suggesting distinct proteolytic processes acting in the normal and tumoral secretomes. The mapping and annotation of cleavage sites in the tumoral secretome suggested functional roles of active proteases in central biological processes related to oncogenesis, such as the processing of growth factors, cleavage of extracellular matrix proteins and the shedding of ectopic domains from the cell surface, some of which may represent novel processed forms of the corresponding proteins. In the context of the tumor microenvironment, these results suggest important biological roles of proteolytic processing in murine melanoma secreted proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:55.474.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andre Zelanis
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2x(13)C,4x(2)H labeled dimethylated residue; acetylated residue; dimethylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-19 08:38:22	ID requested
1	2018-10-02 07:14:44	announced
2	2018-11-04 13:26:20	announced	Updated publication reference for PubMed record(s): 30267877.
⏵ 3	2024-10-22 04:46:00	announced	2024-10-22: Updated project metadata.

Liberato T, Fukushima I, Kitano ES, Serrano SMT, Chammas R, Zelanis A, Proteomic profiling of the proteolytic events in the secretome of the transformed phenotype of melanocyte-derived cells using Terminal Amine Isotopic Labeling of Substrates. J Proteomics, 192():291-298(2019) [pubmed]

10.1016/j.jprot.2018.09.010;

curator keyword: Biological

submitter keyword: cancer, proteomics,Keywords: secretome, TAILS, melanoma, N-terminomics

André Zelanis
contact affiliation	Laboratory of Functional Proteomics - Department of Science and Technology - Federal University of São Paulo (UNIFESP), Sao Jose dos Campos-SP, Brazil Country: Brazil
contact email	zelanis@gmail.com
lab head
Andre Zelanis
contact affiliation	Laboratory of Functional Proteomics - Department of Science and Technology - Federal University of São Paulo (UNIFESP), Sao Jose dos Campos-SP, Brazil
contact email	zelanis@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010169

PRIDE project URI

• PRIDE
  1. PXD010169
     1. Label: PRIDE project
     2. Name: Proteomic profiling of the proteolytic events in the secretome of the transformed phenotype of melanocyte-derived cells using Terminal Amine Isotopic Labeling of Substrates