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PXD010159

PXD010159 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins
DescriptionThe human genome consists of considerable portions derived from retroviruses typically inherited already for millions of years. So-called human endogenous retroviruses (HERVs) are usually severely mutated, yet some coding-competent HERV sequences exist. The HERV-K(HML-2) group includes evolutionarily young proviruses that still encode typical retroviral proteins. HERV-K(HML-2) has been implicated in various human diseases because transcription is often upregulated and encoded proteins are known to affect cell biology. HERV-K(HML-2) protease has received little attention so far. With findings for Human Immunodeficiency Virus (HIV) protease in mind we set out to identify human cellular proteins being substrates of HERV-K(HML-2) protease employing a modified Terminal Amine Isotopic Labeling of Substrates (TAILS) procedure. Thousands of significantly processed human proteins were revealed by TAILS and we could verify cleavage of a majority of selected human proteins in vitro. Our analysis suggests that hundreds, if not thousands of cellular proteins are potential substrates of HERV-K(HML-2) protease. As identified proteins participate in diverse, often disease-relevant cellular processes, it is conceivable that expression of HERV-K(HML-2) protease has functional consequences for cell biology and thus development of human diseases. Endogenous retrovirus-encoded protease may also be relevant for disease development in species other than human.
HostingRepositoryPRIDE
AnnounceDate2019-09-02
AnnouncementXMLSubmission_2019-09-02_05:44:15.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOliver Schilling
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-06-19 00:16:50ID requested
12019-09-02 05:44:16announced
Publication List
Rigogliuso G, Biniossek ML, Goodier JL, Mayer B, Pereira GC, Schilling O, Meese E, Mayer J, A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins. Mob DNA, 10():36(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: N-terminomics, protease
Contact List
Oliver Schilling
contact affiliationPD Dr. Oliver Schilling Heisenberg Fellow Institute of Molecular Medicine and Cell Research University of Freiburg Stefan-Meier-Str. 17, Room 02 027 D-79104 Freiburg, Germany +49 761 203 9615 oliver.schilling@mol-med.uni-freiburg.de
contact emailoliver.schilling@mol-med.uni-freiburg.de
lab head
Oliver Schilling
contact affiliationUniversity of Freiburg
contact emailoliver.schilling@mol-med.uni-freiburg.de
dataset submitter
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Dataset FTP location
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