PXD010159

PXD010159 is an original dataset announced via ProteomeXchange.

Title	A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins
Description	The human genome consists of considerable portions derived from retroviruses typically inherited already for millions of years. So-called human endogenous retroviruses (HERVs) are usually severely mutated, yet some coding-competent HERV sequences exist. The HERV-K(HML-2) group includes evolutionarily young proviruses that still encode typical retroviral proteins. HERV-K(HML-2) has been implicated in various human diseases because transcription is often upregulated and encoded proteins are known to affect cell biology. HERV-K(HML-2) protease has received little attention so far. With findings for Human Immunodeficiency Virus (HIV) protease in mind we set out to identify human cellular proteins being substrates of HERV-K(HML-2) protease employing a modified Terminal Amine Isotopic Labeling of Substrates (TAILS) procedure. Thousands of significantly processed human proteins were revealed by TAILS and we could verify cleavage of a majority of selected human proteins in vitro. Our analysis suggests that hundreds, if not thousands of cellular proteins are potential substrates of HERV-K(HML-2) protease. As identified proteins participate in diverse, often disease-relevant cellular processes, it is conceivable that expression of HERV-K(HML-2) protease has functional consequences for cell biology and thus development of human diseases. Endogenous retrovirus-encoded protease may also be relevant for disease development in species other than human.
HostingRepository	PRIDE
AnnounceDate	2019-09-02
AnnouncementXML	Submission_2019-09-02_05:44:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Schilling
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-19 00:16:50	ID requested
⏵ 1	2019-09-02 05:44:16	announced

curator keyword: Biological

submitter keyword: N-terminomics, protease

Oliver Schilling
contact affiliation	PD Dr. Oliver Schilling Heisenberg Fellow Institute of Molecular Medicine and Cell Research University of Freiburg Stefan-Meier-Str. 17, Room 02 027 D-79104 Freiburg, Germany +49 761 203 9615 oliver.schilling@mol-med.uni-freiburg.de
contact email	oliver.schilling@mol-med.uni-freiburg.de
lab head
Oliver Schilling
contact affiliation	University of Freiburg
contact email	oliver.schilling@mol-med.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010159
     1. Label: PRIDE project
     2. Name: A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins