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PXD010152

PXD010152 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Novel Two-Stage Tandem Mass Spectrometry Approach and Scoring Scheme for the Identification of O-GlcNAc Modified Peptides
DescriptionThe modification of serine and threonine residues in proteins by a single N-acetylglucosamine (O-GlcNAc) residue is an emerging post-translational modification (PTM) with broad biological implications. However, the systematic or large-scale analysis of this PTM is hampered by several factors, including low stoichiometry and the lability of the O-glycosidic bond during tandem mass spectrometry. Using a library of 72 synthetic glycopeptides, we developed a two-stage tandem MS approach consisting of pulsed Q dissociation (PQD) for O-GlcNAc peptide detection and electron transfer dissociation (ETD) for identification and site localization. Based on a set of O-GlcNAc specific fragment ions, we further developed a score (OScore) that discriminates O-GlcNAc peptide spectra from spectra of unmodified peptides with 95% sensitivity and >99% specificity. Integrating the OScore into the two-stage LC-MS/MS approach detected O-GlcNAc peptides in the low fmol range and at 10-fold better sensitivity than a single data-dependent ETD tandem MS experiment.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:45:46.897.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHannes Hahne
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListO-(N-acetylamino)glucosyl-L-threonine; O-(N-acetylamino)glucosyl-L-serine
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-06-18 06:02:09ID requested
12018-06-18 06:29:57announced
22018-06-20 00:31:13announcedUpdated project metadata.
32024-10-22 04:45:47announced2024-10-22: Updated project metadata.
Publication List
Hahne H, Kuster B, A novel two-stage tandem mass spectrometry approach and scoring scheme for the identification of O-GlcNAc modified peptides. J Am Soc Mass Spectrom, 22(5):931-42(2011) [pubmed]
10.1007/s13361-011-0107-y;
Keyword List
curator keyword: Technical
submitter keyword: O-GlcNAc
Contact List
Bernhard Kuster
contact affiliationTechnische Universität München Chair of Proteomics and Bioanalytics Freising, Germany
contact emailkuster@tum.de
lab head
Hannes Hahne
contact affiliationOmicScouts GmbH
contact emailhannes.hahne@omicscouts.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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