PXD010152 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Novel Two-Stage Tandem Mass Spectrometry Approach and Scoring Scheme for the Identification of O-GlcNAc Modified Peptides |
Description | The modification of serine and threonine residues in proteins by a single N-acetylglucosamine (O-GlcNAc) residue is an emerging post-translational modification (PTM) with broad biological implications. However, the systematic or large-scale analysis of this PTM is hampered by several factors, including low stoichiometry and the lability of the O-glycosidic bond during tandem mass spectrometry. Using a library of 72 synthetic glycopeptides, we developed a two-stage tandem MS approach consisting of pulsed Q dissociation (PQD) for O-GlcNAc peptide detection and electron transfer dissociation (ETD) for identification and site localization. Based on a set of O-GlcNAc specific fragment ions, we further developed a score (OScore) that discriminates O-GlcNAc peptide spectra from spectra of unmodified peptides with 95% sensitivity and >99% specificity. Integrating the OScore into the two-stage LC-MS/MS approach detected O-GlcNAc peptides in the low fmol range and at 10-fold better sensitivity than a single data-dependent ETD tandem MS experiment. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:45:46.897.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hannes Hahne |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | O-(N-acetylamino)glucosyl-L-threonine; O-(N-acetylamino)glucosyl-L-serine |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-18 06:02:09 | ID requested | |
1 | 2018-06-18 06:29:57 | announced | |
2 | 2018-06-20 00:31:13 | announced | Updated project metadata. |
⏵ 3 | 2024-10-22 04:45:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
Hahne H, Kuster B, A novel two-stage tandem mass spectrometry approach and scoring scheme for the identification of O-GlcNAc modified peptides. J Am Soc Mass Spectrom, 22(5):931-42(2011) [pubmed] |
10.1007/s13361-011-0107-y; |
Keyword List
curator keyword: Technical |
submitter keyword: O-GlcNAc |
Contact List
Bernhard Kuster |
contact affiliation | Technische Universität München Chair of Proteomics and Bioanalytics Freising, Germany |
contact email | kuster@tum.de |
lab head | |
Hannes Hahne |
contact affiliation | OmicScouts GmbH |
contact email | hannes.hahne@omicscouts.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010152
- Label: PRIDE project
- Name: A Novel Two-Stage Tandem Mass Spectrometry Approach and Scoring Scheme for the Identification of O-GlcNAc Modified Peptides