PXD010145

PXD010145 is an original dataset announced via ProteomeXchange.

Title	Acid bone lysates: what we eliminate by preparing demineralized bone matrix
Description	Demineralized bone matrix is a widely used allograft where not only the inorganic mineral but also embedded growth factors are removed by hydrochloric acid. The cellular response to the growth factors released during the preparation of demineralized bone matrix, however, has not been studied. Here we investigated the in vitro impact of acid bone lysates (ABL) prepared from porcine cortical bone chips on oral fibroblasts. Proteomic analysis of ABL revealed a large spectrum of bone-derived proteins including TGF-β1. Whole genome microarrays and RT-PCR together with the pharmacologic blocking of TGF-β receptor type I kinase with SB431542 showed that ABL activates the TGF-β target genes interleukin 11, proteoglycan 4, and NADPH oxidase 4. Interleukin 11 expression was confirmed at the protein level by ELISA. Immunofluorescence and Western blot showed the nuclear localization of Smad2/3 and increased phosphorylation of Smad3 with ABL, respectively. This effect was independent of whether ABL was prepared from mandible, calvaria or tibia. ABL prepared with 0.1 N HCL was most effective compared to lysates obtained with 0.01 N and also 1.0 N HCl. These results demonstrate that TGF-β is a major growth factor that is removed upon the preparation of demineralized bone matrix.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:51.992.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010145
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Goran Mitulović
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-18 00:34:37	ID requested
1	2018-11-04 13:33:31	announced
⏵ 2	2024-10-22 04:46:00	announced	2024-10-22: Updated project metadata.

10.6019/PXD010145;

10.1038/s41598-018-34418-3;

Strauss FJ, St, ä, hli A, Beer L, Mitulovi, ć G, Gilmozzi V, Haspel N, Schwab G, Gruber R, signalling in human oral fibroblasts. Sci Rep, 8(1):16065(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: growth factors,bone matrix, acid bone lysates

Goran Mitulović
contact affiliation	Head Proteomics Core Facility Medical University of Vienna Clinical Department of Laboratory Medicine
contact email	goran.mitulovic@meduniwien.ac.at
lab head
Goran Mitulović
contact affiliation	Medizinische Universität Wien
contact email	goran.mitulovic@meduniwien.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010145
     1. Label: PRIDE project
     2. Name: Acid bone lysates: what we eliminate by preparing demineralized bone matrix