PXD010132

PXD010132 is an original dataset announced via ProteomeXchange.

Title	20S proteasome digestion of HeLa cell extracts
Description	The 20S proteasome is responsible for the catalytic activity of all proteasome complexes. Structural constraints mean that only unfolded, extended polypeptide chains may enter the catalytic core of the 20S proteasome. Here we conducted a comprehensive analysis of the 20S CP substrates in-vitro. We revealed that the 20S CP substrates are highly structually disordered. The 20S proteasome substrate group, termed 20S-IDPome are characterized by having significantly more protein binding partners, more post-translational modification sites and are highly enriched for RNA binding proteins. Remarkably, we found that low complexity proteins with prion-like domain which interact with GR or PR di-peptide repeats are the most preferential 20S proteasome substrates. Our finding suggests roles of the 20S proteasome in gene transcription and formation of phase-separated granules.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:39.297.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nadav Myers
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-14 08:52:43	ID requested
1	2018-07-30 02:27:30	announced
⏵ 2	2024-10-22 04:45:46	announced	2024-10-22: Updated project metadata.

Myers N, Olender T, Savidor A, Levin Y, Reuven N, Shaul Y, The Disordered Landscape of the 20S Proteasome Substrates Reveals Tight Association with Phase Separated Granules. Proteomics, 18(21-22):e1800076(2018) [pubmed]

10.1002/pmic.201800076;

curator keyword: Biological

submitter keyword: 20S proteasome digestion, heat soluble proteins

Yosef Shaul
contact affiliation	Department of Molecular Genetics Weizmann Institute of Science
contact email	Yosef.Shaul@weizmann.ac.il
lab head
Nadav Myers
contact affiliation	Postdoctoral fellow
contact email	nadav.myers@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD010132

PRIDE project URI

• PRIDE
  1. PXD010132
     1. Label: PRIDE project
     2. Name: 20S proteasome digestion of HeLa cell extracts