PXD010130

PXD010130 is an original dataset announced via ProteomeXchange.

Title	repaired shells of Pinctada fucata LC-MSMS
Description	Shells of pearl oyster are natural biominerals with remarkable properties and can be repaired after damage, which are regulated by biomacromolcules especially shell matrix proteins (SMPs). Identifying SMPs is critical for further understanding the process. Although proteomic methods have been used to reveal the complex protein mixture in mature shells, the proteomics of repaired shells after shell damage have not been reported before. Here, we studied the SMPs of the repaired shells (prismatic layers) 5-10 days after shell damage in Pinctada fucata by integrating transcriptomics and proteomics and compared the microstructure difference between repaired and mature shells. Although the repaired shells are calcite, similar to mature shells, the microstructure of repaired shells has holes in the center of prisms with irregular and curved edges. In total, we found 49 SMPs from the repaired shells including some proteins only existing in mature nacreous layers. Peroxidase-like protein and beta-N-acetylhexosaminidase may be important players in repaired shells. In addition, they have the capability to affect CaCO3 crystallization process in vitro, altering the packing and reducing the crystallinity of crystals. This study could improve our understanding of shell repair process and lay the foundation for studying SMPs-controlled biomineralization.
HostingRepository	PRIDE
AnnounceDate	2019-03-26
AnnouncementXML	Submission_2019-03-26_02:14:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Chen
SpeciesList	scientific name: Pinctada fucata; NCBI TaxID: 50426;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-14 08:31:11	ID requested
⏵ 1	2019-03-26 02:14:56	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Pinctada fucata, repaired shells, proteomics

Rongqing Zhang
contact affiliation	Tsinghua university
contact email	razhang@mail.tsinghua.edu.cn
lab head
Yan Chen
contact affiliation	Tsinghua university
contact email	chenyan15@mails.tsinghua.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD010130

PRIDE project URI

• PRIDE
  1. PXD010130
     1. Label: PRIDE project
     2. Name: repaired shells of Pinctada fucata LC-MSMS