PXD010126

PXD010126 is an original dataset announced via ProteomeXchange.

Title	Multifaceted stoichiometry control of bacterial operons revealed by data-independent acquisition mass spectrometry
Description	Genes clustered into polycistronic operons was thought a characteristic of bacteria and many other species. More than half protein-coding genes are organized in polycistronic operons composed of two or more than ten genes in bacterial genomes. Although the structure of operons have been studied precisely, how the member genes within operon maintain their stoichiometry expression is remain unknown. Using a highly accurate label-free absolute quantification method DIA (data-independent acquisition), we present a global analysis of Escherichia coli proteome, quantified 1607 proteins, including 59.1% of the known polycistronic operons. We found shorter operons tend to be more tightly controlled than longer operons, and those operons for metabolic pathways are less controlled for stoichiometry balance than those operons for protein complexes. Our results thus reveal the two-level regulation mode involving transcription and translation of operons would balance the stoichiometry expression of genes in polycistronic operons in different time-scale.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:44.479.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Zhao
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-14 02:27:38	ID requested
1	2019-11-12 11:31:46	announced
⏵ 2	2024-10-22 04:45:48	announced	2024-10-22: Updated project metadata.

10.3389/fgene.2019.00473;

Zhao J, Zhang H, Qin B, Nikolay R, He QY, Spahn CMT, Zhang G, Multifaceted Stoichiometry Control of Bacterial Operons Revealed by Deep Proteome Quantification. Front Genet, 10():473(2019) [pubmed]

curator keyword: Biological

submitter keyword: operons, mass spectrometry,faceted stoichiometry control

Gong Zhang
contact affiliation	Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China
contact email	zhanggong-uni@qq.com
lab head
Jing Zhao
contact affiliation	Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan Univrsity, Guangzhou510632, China
contact email	qzhaojing@jnu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD010126

PRIDE project URI

• PRIDE
  1. PXD010126
     1. Label: PRIDE project
     2. Name: Multifaceted stoichiometry control of bacterial operons revealed by data-independent acquisition mass spectrometry